Dual-label STED nanoscopy of living cells using photochromism
| Autor: | Katrin I. Willig, Andre C. Stiel, Stefan Jakobs, Tanja Brakemann, Stefan W. Hell |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Light
Photochemistry Green Fluorescent Proteins Bioengineering Nanotechnology Green fluorescent protein Photochromism Microscopy Chlorocebus aethiops Animals General Materials Science Vero Cells Fluorescent Dyes Microscopy Confocal Chemistry Mechanical Engineering STED microscopy Protein species General Chemistry Condensed Matter Physics Single excitation Superresolution Fluorescence Microscopy Fluorescence Biophysics |
| Zdroj: | Nano Letters |
| Popis: | We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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