Multifunctional optofluidic lab-on-chip platform for Raman and fluorescence spectroscopic microfluidic analysis
| Autor: | Genni Testa, Immacolata Angelica Grimaldi, Gianluca Persichetti, Romeo Bernini |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Riboflavin Biomedical Engineering Bioengineering 02 engineering and technology Spectrum Analysis Raman 01 natural sciences Biochemistry law.invention symbols.namesake Optics Limit of Detection law Lab-On-A-Chip Devices Microscopy sensori Spectroscopy Detection limit Total internal reflection Spectrometer business.industry 010401 analytical chemistry General Chemistry Microfluidic Analytical Techniques Lab-on-a-chip 021001 nanoscience & nanotechnology Chip 0104 chemical sciences Microscopy Fluorescence Models Chemical Spettroscopia Raman. fluorescenza optofluidica symbols Eosine Yellowish-(YS) 0210 nano-technology Raman spectroscopy business |
| Zdroj: | Lab on a chip 17 (2017): 2631–2639. doi:10.1039/c7lc00460e info:cnr-pdr/source/autori:Persichetti, G.; Grimaldi, I. A.; Testa, G.; Bernini, R./titolo:Multifunctional optofluidic lab-on-chip platform for Raman and fluorescence spectroscopic microfluidic analysis/doi:10.1039%2Fc7lc00460e/rivista:Lab on a chip (Print)/anno:2017/pagina_da:2631/pagina_a:2639/intervallo_pagine:2631–2639/volume:17 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c7lc00460e |
| Popis: | A multifunctional lab-on-a-chip platform for spectroscopic analysis of liquid samples based on an optofluidic jet waveguide is reported. The optofluidic detection scheme is achieved through the total internal reflection arising in a liquid jet of only 150 mu m diameter, leading to highly efficient signal excitation and collection. This results in an optofluidic chip with an alignment-free spectroscopic detection scheme, which avoids any background from the sample container. This platform has been designed for multiwavelength fluorescence and Raman spectroscopy. The chip integrates a recirculation system that reduces the required sample volume. The evaluation of the device performance has been accomplished by means of fluorescence measurements performed on eosin Y in water solutions, achieving a limit of detection of 33 pM. The sensor has been applied in Raman spectroscopy of water-ethanol solutions, leading to a limit of detection of 0.18%. As additional application, analysis of riboflavin using fluorescence detection demonstrates the possibility of detecting this vitamin at the 560 pM level (0.21 ng l(-1)). Although measurements have been performed by means of a compact and low-cost spectrometer, in both cases the microjet optofluidic chip achieved similar performances if not better than high-end benchtop based laboratory equipment. This approach paves the way towards portable lab-on-a-chip devices for high sensitivity environmental and biochemical sensing, using optical spectroscopy. |
| Databáze: | OpenAIRE |
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