Recombinant DNA vaccine encoding multiple domains related to inhibition of neurite outgrowth: a potential strategy for axonal regeneration
| Autor: | Gang Xu, Chao-Yang Wang, Sohail Ahmed, Feng-Gang Yu, Zhi-Cheng Xiao, Li Sun, Samuel David, Xue-gang Luo, Duyu Nie, Ju-Tao Chen |
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| Rok vydání: | 2004 |
| Předmět: |
Nogo Proteins
Encephalomyelitis Autoimmune Experimental Biology Motor Activity Biochemistry Epitope law.invention DNA vaccination Cellular and Molecular Neuroscience Myelin law medicine Neurites Vaccines DNA Animals Humans Spinal Cord Injuries Myelin-associated glycoprotein Regeneration (biology) Experimental autoimmune encephalomyelitis Immunotherapy Active Tenascin Recovery of Function medicine.disease Axons Cell biology Nerve Regeneration Protein Structure Tertiary Rats Disease Models Animal Myelin-Associated Glycoprotein medicine.anatomical_structure nervous system Rats Inbred Lew COS Cells Recombinant DNA Female Neuroscience Myelin Proteins |
| Zdroj: | Journal of neurochemistry. 91(4) |
| ISSN: | 0022-3042 |
| Popis: | Myelin-derived proteins, such as tenascin-R (TN-R), myelin associate glycoprotein (MAG), and Nogo-A, inhibit the CNS regeneration. By targeting specifically the inhibitory epitopes, we have investigated whether vaccination with a recombinant DNA molecule encoding multiple domains of myelin inhibitors may be useful in CNS repair. We show here that the recombinant DNA vaccine is able to activate the immune system but does not induce experimental autoimmune encephalomyelitis (EAE) in Lewis rats. Importantly, it promotes axonal regeneration in a spinal cord injury model. Thus, the application of DNA vaccine, encoding multiple specific domains of major inhibitory proteins and/or their receptors, provides another promising approach to overcome the inhibitory barriers during CNS regeneration. |
| Databáze: | OpenAIRE |
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