Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Autor: Srujan Marepally, Yogapriya Periyasamy, Kanimozhi Subramaniyam, Ajay Kumar Dhyani, Porkizhi Arjunan, Aruna Mohan, Gokulnath Mahalingam
Rok vydání: 2022
Předmět:
Zdroj: Journal of Visualized Experiments.
ISSN: 1940-087X
DOI: 10.3791/62407
Popis: In recent years, chemically modified messenger RNA (mRNA) has emerged as a potent nucleic acid molecule for developing a wide range of therapeutic applications, including a novel class of vaccines, protein replacement therapies, and immune therapies. Among delivery vectors, lipid nanoparticles are found to be safer and more effective in delivering RNA molecules (e.g., siRNA, miRNA, mRNA) and a few products are already in clinical use. To demonstrate lipid nanoparticle-mediated mRNA delivery, we present an optimized protocol for the synthesis of functional me1Ψ-UTP modified eGFP mRNA, the preparation of cationic liposomes, the electrostatic complex formation of mRNA with cationic liposomes, and the evaluation of transfection efficiencies in mammalian cells. The results demonstrate that these modifications efficiently improved the stability of mRNA when delivered with cationic liposomes and increased the eGFP mRNA translation efficiency and stability in mammalian cells. This protocol can be used to synthesize the desired mRNA and transfect with cationic liposomes for target gene expression in mammalian cells.
Databáze: OpenAIRE