Controlled Delivery of Stem Cell-Derived Trophic Factors Accelerates Kidney Repair After Renal Ischemia-Reperfusion Injury in Rats
Autor: | Doo Sang Kim, Brian Shing, In Kap Ko, Kyung Hyun Moon, Hyung Eun Yim, James J. Yoo, Zachary P Atala, Sunil George, Hyun Chul Chung, Michael W. Kim, Ji Hyun Kim |
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Rok vydání: | 2019 |
Předmět: |
Male
0301 basic medicine Placenta Apoptosis Pharmacology Placenta cord banking Kidney Rats Nude 03 medical and health sciences 0302 clinical medicine Pregnancy Tissue Engineering and Regenerative Medicine medicine Animals lcsh:QH573-671 Conditioned medium Cell Proliferation lcsh:R5-920 Platelet-Rich Plasma lcsh:Cytology business.industry Stem Cells Regeneration (biology) Acute kidney injury Cell Biology General Medicine medicine.disease Controlled release Cell Hypoxia Rats Drug delivery systems 3. Good health Transplantation 030104 developmental biology medicine.anatomical_structure Erythropoietin Culture Media Conditioned Reperfusion Injury Female Kidney regeneration Stem cell lcsh:Medicine (General) business Gels 030217 neurology & neurosurgery Developmental Biology medicine.drug |
Zdroj: | Stem Cells Translational Medicine, Vol 8, Iss 9, Pp 959-970 (2019) Stem Cells Translational Medicine |
ISSN: | 2157-6580 2157-6564 |
Popis: | Renal disease is a worldwide health issue. Besides transplantation, current therapies revolve around dialysis, which only delays disease progression but cannot replace other renal functions, such as synthesizing erythropoietin. To address these limitations, cell-based approaches have been proposed to restore damaged kidneys as an alternative to current therapies. Recent studies have shown that stem cell-derived secretomes can enhance tissue regeneration. However, many growth factors undergo rapid degradation when they are injected into the body in a soluble form. Efficient delivery and controlled release of secreting factors at the sites of injury would improve the efficacy in tissue regeneration. Herein, we developed a gel-based delivery system for controlled delivery of trophic factors in the conditioned medium (CM) secreted from human placental stem cells (HPSCs) and evaluated the effect of trophic factors on renal regeneration. CM treatment significantly enhanced cell proliferation and survival in vitro. Platelet-rich plasma (PRP) was used as a delivery vehicle for CM. Analysis of the release kinetics demonstrated that CM delivery through the PRP gel resulted in a controlled release of the factors both in vitro and in vivo. In an acute kidney injury model in rats, functional and structural analysis showed that CM delivery using the PRP gel system into the injured kidney minimized renal tissue damage, leading to a more rapid functional recovery when compared with saline, CM, or vehicle only injection groups. These results suggest that controlled delivery of HPSC-derived trophic factors may provide efficient repair of renal tissue injury. Stem Cells Translational Medicine 2019;8:959–970 |
Databáze: | OpenAIRE |
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