Sexually Dimorphic Neuropeptide B Neurons in Medaka Exhibit Activated Cellular Phenotypes Dependent on Estrogen
| Autor: | Kataaki Okubo, Mikoto Nakajo, Chie Umatani, Keisuke Matsumoto, Yukiko Kikuchi, Hitoshi Ozawa, Towako Hiraki-Kajiyama, Shinji Kanda, Yoshitaka Oka |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Neuropeptide B medicine.drug_class Oryzias Population 030209 endocrinology & metabolism 03 medical and health sciences Sexual Behavior Animal 0302 clinical medicine Endocrinology Internal medicine medicine Animals education Cell Nucleus Neurons education.field_of_study Sexual differentiation biology Estradiol Neurogenesis Neuropeptides Brain biology.organism_classification Sexual dimorphism 030104 developmental biology medicine.anatomical_structure Phenotype nervous system Estrogen Female Endoplasmic Reticulum Rough Nucleus |
| Zdroj: | Endocrinology. 160(4) |
| ISSN: | 1945-7170 |
| Popis: | Brain and behavior of teleosts are highly sexually plastic throughout life, yet the underlying neural mechanisms are largely unknown. On examining brain morphology in the teleost medaka (Oryzias latipes), we identified distinctively large neurons in the magnocellular preoptic nucleus that occurred much more abundantly in females than in males. Examination of sex-reversed medaka showed that the sexually dimorphic abundance of these neurons is dependent on gonadal phenotype, but independent of sex chromosome complement. Most of these neurons in females, but none in males, produced neuropeptide B (Npb), whose expression is known to be estrogen-dependent and associated with female sexual receptivity. In phenotypic analysis, the female-specific Npb neurons had a large euchromatic nucleus with an abundant cytoplasm containing plentiful rough endoplasmic reticulum, exhibited increased overall transcriptional activity, and typically displayed a spontaneous regular firing pattern. These phenotypes, which are probably indicative of cellular activation, were attenuated by ovariectomy and restored by estrogen replacement. Furthermore, the population of Npb-expressing neurons emerged in adult males treated with estrogen, not through frequently occurring neurogenesis in the adult teleost brain, but through the activation of preexisting, quiescent male counterpart neurons. Collectively, our results demonstrate that the morphological, transcriptional, and electrophysiological phenotypes of sexually dimorphic preoptic Npb neurons are highly dependent on estrogen and can be switched between female and male patterns. These properties of the preoptic Npb neurons presumably underpin the neural mechanism for sexual differentiation and plasticity of brain and behavior in teleosts. |
| Databáze: | OpenAIRE |
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