Isolation and Characterization of Bacteria that Produce Polyhydroxybutyrate Depolymerases†
| Autor: | L F Hanne, L. L. Kirk, Emily A. Egusa, MyLo L. Thao, Daniel J. Edwards |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
QH301-705.5
Tips & Tools 02 engineering and technology macromolecular substances engineering.material General Biochemistry Genetics and Molecular Biology Education Polyhydroxybutyrate Environmental Microbiology Food science Polymer Biodegradation Biology (General) Depolymerase Assay lcsh:QH301-705.5 lcsh:LC8-6691 General Immunology and Microbiology biology LC8-6691 Poly(3-hydroxybutyrate) (PHB) Degraders lcsh:Special aspects of education Chemistry 05 social sciences technology industry and agriculture 050301 education Bioplastic Degradation 021001 nanoscience & nanotechnology Isolation (microbiology) biology.organism_classification Antibiotic production Special aspects of education Carbon storage lcsh:Biology (General) biology.protein engineering Exoenzyme Plastic waste lipids (amino acids peptides and proteins) Microbiology Laboratory Exercise Biopolymer 0210 nano-technology General Agricultural and Biological Sciences 0503 education Bacteria |
| Zdroj: | Journal of Microbiology & Biology Education Journal of Microbiology & Biology Education, Vol 19, Iss 3 (2019) Journal of Microbiology & Biology Education, Vol 19, Iss 3 (2018) |
| ISSN: | 1935-7885 1935-7877 |
| Popis: | Isolation of environmental bacterial strains with diverse metabolic properties such as antibiotic production, sulfide oxidation, and the ability to use different nutrient sources is a common undergraduate lab experience. This article describes a useful protocol for the detection and isolation of bacteria that can degrade the common carbon storage biopolymer poly (3-hydroxybutyrate) (PHB). The procedure utilizes a low nutrient media supplemented with insoluble PHB that allows for the easy, visible detection of strains that produce extracellular PHB degrading enzymes. This method can be used to determine the percent of culturable bacteria from various environments that can degrade PHB. Further, PHB degrading exoenzyme activity can be monitored from pure cultures by: (1) Growing cells in broth, (2) inducing with PHB, and (3) measuring activity of supernatant with a UV/Vis spectrophotometer. The enzymatic breakdown of PHB presents an opportunity to expose students to the concept of using biodegradable plastics as a solution to the global plastic waste problem. |
| Databáze: | OpenAIRE |
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