Thyrotrophin regulation of apical and basal exocytosis of thyroglobulin by porcine thyroid monolayers
| Autor: | Odile Chabaud, Marianne Chambard, Jean Mauchamp, S. Gonzalvez, D. Depetris, D. Gruffat |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
endocrine system
medicine.medical_specialty Cholera Toxin endocrine system diseases Swine medicine.medical_treatment Thyroid Gland 8-Bromo Cyclic Adenosine Monophosphate Thyrotropin Stimulation Biology medicine.disease_cause Thyroglobulin Exocytosis Endocrinology Internal medicine medicine Animals Secretion Molecular Biology Cells Cultured Dose-Response Relationship Drug Cholera toxin Thyroid Culture Media Kinetics medicine.anatomical_structure Cell culture hormones hormone substitutes and hormone antagonists Endocrine gland |
| Zdroj: | Journal of molecular endocrinology. 4(3) |
| ISSN: | 0952-5041 |
| Popis: | Exocytosis, the ultimate step in thyroglobulin secretion, has been studied in porcine thyroid cells cultured in monolayers on the permeable bottom of culture chambers. We have previously demonstrated, using this culture system, that apical secretion accounts for 85–95% of total secretion of newly synthesized thyroglobulin. When cells were cultured for several days with bovine TSH (25 μU/ml) in the basal medium, the rate of glycoprotein accumulation in the upper compartment was three times higher than that in the absence of TSH. In contrast, the rate of thyroglobulin released into the basal medium (5–15% of total secreted thyroglobulin) appeared unmodified by chronic TSH stimulation. To investigate the effect of acute TSH stimulation on thyroglobulin exocytosis in the apical and basal compartments, pulse-chase experiments were carried out with the same culture system. The release of radiolabelled thyroglobulin (1·5-h pulse) into the apical medium was increased threefold during the 2-h chase period under TSH stimulation. The radiolabelled thyroglobulin released into the basal medium was increased only 1·5- to 2-fold, and stimulation disappeared after 1 h. The effect of TSH was maximal when the chase medium contained 50 μU TSH/ml. However, cells cultured for several days in the presence of 25 μU TSH/ml before the pulse-chase experiment, appeared desensitized to acute TSH stimulation. Similar responses were observed when the chase medium contained 8-chloro-cyclic AMP or cholera toxin. This study provides another example of the pleiotropic effect of TSH, mediated by cyclic AMP, on the sequential steps of thyroglobulin gene expression in cultured thyroid cells in which the polar character of the epithelial cells is well preserved. |
| Databáze: | OpenAIRE |
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