Replication potentials of vif variant viruses generated from monkey cell-tropic HIV-1 derivative clones NL-DT5/NL-DT5R
| Autor: | Tomoki Yamashita, Akio Adachi, Kazuki Hatcho, Kazuya Kamada, Masako Nomaguchi |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
viruses
Immunology APOBEC-3G Deaminase Biology medicine.disease_cause Virus Replication Microbiology Virus Cell Line Cytosine Deaminase Cytidine Deaminase medicine Animals Humans Cloning Molecular Gene APOBEC3G Tropism Infectivity Genes vif virus diseases Haplorhini biochemical phenomena metabolism and nutrition Simian immunodeficiency virus Virology HIV Reverse Transcriptase Infectious Diseases Viral replication Mutation HIV-1 |
| Zdroj: | Microbes and infection. 10(10-11) |
| ISSN: | 1286-4579 |
| Popis: | To obtain monkey-tropic viruses that are more closely related to HIV-1 than the original NL-DT5/NL-DT5R clones, we constructed six vif-chimeric and two site-specific vif-mutant viruses, and examined their growth ability. Different from NL-DT5/NL-DT5R, these viruses did not grow in monkey cells. We monitored the capability of the mutants to antagonize monkey APOBEC3G/F by single-cycle infectivity assays. They counteracted poorly or not at all the action of the APOBEC3G/F. Our results have indicated that the native SIVmac Vif is required to overcome the species barrier against HIV-1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect