Effects of administration of progenipoietin 1, Flt-3 ligand, granulocyte colony-stimulating factor, and pegylated granulocyte-macrophage colony-stimulating factor on dendritic cell subsets in mice
| Autor: | Ken Shortman, Woulfe Susan L, Joanne Pooley, Hubertus Hochrein, Meredith O'Keeffe, David Vremec, Robert W. Evans |
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| Rok vydání: | 2002 |
| Předmět: |
Male
CD8 Antigens medicine.medical_treatment Immunology Biology Granulocyte Biochemistry Immunophenotyping Polyethylene Glycols Interferon-gamma Mice Adjuvants Immunologic Colony-Stimulating Factors Interferon Granulocyte Colony-Stimulating Factor medicine Animals Humans Antigen-presenting cell Dose-Response Relationship Drug Granulocyte-Macrophage Colony-Stimulating Factor Membrane Proteins Dendritic Cells Cell Biology Hematology Dendritic cell Interleukin-12 Recombinant Proteins Granulocyte colony-stimulating factor Mice Inbred C57BL Granulocyte macrophage colony-stimulating factor medicine.anatomical_structure Cytokine Interleukin 12 Cytokines Lymphocyte Culture Test Mixed Cell Division Spleen medicine.drug |
| Zdroj: | Blood. 99:2122-2130 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v99.6.2122 |
| Popis: | We studied the effects of administration of several cytokines, including progenipoietin-1 (ProGP-1), Flt-3 ligand (FL), granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor in a pegylated form (pGM-CSF), on dendritic cell (DC) populations in mouse spleen. ProGP-1 produced the most striking increase in overall DC numbers, apparently more than its constituent FL and G-CSF components. However, the expansion in DC numbers was strongly subpopulation selective, with ProGP-1 and FL producing selective expansion of CD8+ DCs, whereas pGM-CSF produced selective expansion of CD8− DCs. Surprising differences were observed between the effects of murine and human recombinant FL preparations on murine DCs. Many of the biologic functions of the DC subpopulations expanded by cytokines remained intact, including the capacity of the ProGP-1– and FL-expanded CD8+ DCs to produce the T-helper-1–biasing cytokine interleukin 12 (IL-12). However, the expanded DCs from all but G-CSF–treated mice were deficient in the ability to make interferon γ, and the CD8+ DCs produced with pGM-CSF treatment had an abrogated capacity to form bioactive IL-12. Such selective expansion of DC populations and alterations in their cytokine-secretion capacity have implications for clinical use of the studied cytokines in immune modulation. |
| Databáze: | OpenAIRE |
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