Cloning, sequencing, chromosomal location, and function of cDNAs encoding an opioid growth factor receptor (OGFr) in humans
| Autor: | Sandra S Allen, Ian S. Zagon, Patricia J. McLaughlin, Michael F. Verderame |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Repetitive Sequences Amino Acid DNA Complementary medicine.drug_class Placenta medicine.medical_treatment Molecular Sequence Data Chromosomes Human Pair 20 Biology Oligodeoxyribonucleotides Antisense OGFr Neuroblastoma Open Reading Frames Fetus Pregnancy Opioid receptor Complementary DNA Tumor Cells Cultured medicine Animals Humans Protein Isoforms Amino Acid Sequence Cloning Molecular Receptor Molecular Biology Peptide sequence General Neuroscience Growth factor Alternative splicing Brain Chromosome Mapping Molecular biology Recombinant Proteins Rats Alternative Splicing Open reading frame Receptors Opioid Female Neurology (clinical) Sequence Alignment Developmental Biology |
| Zdroj: | Brain Research. 856:75-83 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/s0006-8993(99)02330-6 |
| Popis: | The native opioid growth factor (OGF), [Met(5)]-enkephalin, is a tonic inhibitory peptide that modulates cell proliferation and tissue organization during development, cancer, cellular renewal, wound healing, and angiogenesis. OGF action is mediated by a receptor mechanism. We have cloned and sequenced cDNAs encoding multiple spliced forms of a human OGF receptor. The open reading frame in the longest cDNA was found to encode a protein of 697 amino acids, and 8 imperfect repeats of 20 amino acids each were a prominent feature. Altogether, five alternatively spliced forms were observed. The cDNA hybridized to mRNA from a variety of normal and neoplastic cells and tissues. Functional studies using antisense oligonucleotides to OGFr demonstrated an enhancement in cell growth. Fluorescent in situ hybridization (FISH) experiments showed the chromosomal location to be 20q13.3. This OGF receptor has no homology to classical opioid receptors. These results provide molecular validity for the interaction of OGF and OGF receptor in the regulation of growth processes in humans. |
| Databáze: | OpenAIRE |
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