Selective localization of interleukin-1 receptor antagonist in eutopic endometrium and endometriotic implants
| Autor: | Jouko Halme, Vicken Sahakian, Stephen Haskill, Joyce Anners |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adult
medicine.medical_specialty Pathology Stromal cell medicine.drug_class Biopsy Endometriosis Biology Endometrium Internal medicine medicine Humans Tissue Distribution Frozen section procedure Staining and Labeling Receptors Interleukin-1 Obstetrics and Gynecology Interleukin medicine.disease Receptor antagonist Immunohistochemistry Endocrinology Interleukin 1 receptor antagonist medicine.anatomical_structure Reproductive Medicine Female |
| Zdroj: | Fertility and Sterility. 60:276-279 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(16)56097-x |
| Popis: | Objectives To determine whether interleukin-1 receptor antagonist (IL-1ra) expression was concordant in eutopic endometrium and endometriotic implants. Design Paired samples of endometrium and endometriotic implants from eight patients with endometriosis were used. Main Outcome Measures Interleukin-1 receptor antagonist was demonstrated immunohisto-chemically on frozen sections of eutopic and ectopic endometria. A sandwich technique with polyclonal rabbit anti-IL-1ra antibody and an avidin-biotin reagent (Vector Laboratories, Inc. Burlingame, CA) was used. Results Seven of eight (88%) eutopic endometrial sections revealed staining of glandular epithelium with complete absence of any staining of the stromal compartment. In the counterpart sections of endometriosis, the glandular as well as the stromal compartments were negative for IL-1ra in all patients. Conclusion These data suggest a differential production of the IL-1ra in eutopic endometrium and endometriotic implants. The potential clinical implications of this finding are discussed. |
| Databáze: | OpenAIRE |
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