| Popis: |
Flavobacterium psychrophilum JIP02/86 of the Flavobacteriaceae family is currently one of the most destructive fish pathogens, causing Rainbow trout fry syndrome in freshwater fishes has an economic impact. Genome features revealed many promising uncharacterised proteins (Hypothetical proteins) available in Flavobacterium psychrophilum JIP02/86. F. psychrophilum contains 13 putative proteases that are probably involved in the virulence or destruction of host tissues. The function prediction of this protein led to the detection of target involved in pathogenicity, was done using Bioinformatics pipeline involves PSI-BLAST for similarity search, InterProScan, TMHMM, and CDD for domain searching, PFP, ProtFun, Pfam and COGNITOR for function prediction, Phyre2 for secondary fold annotation, and Meta server for 3D homology model search. Through this pipeline, we have predicted 450 hypothetical proteins out of that “TonB” (outer membrane receptor) may involve in pathogenicity of the organism, “MCE” (Mammalian Cell Entry Domain) can enter in a mammalian cell which resists the entry of microbes in the intracellular environment of mammalian cell also known as essential virulence. This study also revealed many other uncharacterised proteins yet explored in Flavobacterium psychrophilum JIP01/86. Moreover, the function of the proteins leads to the various aspects of the research specifically for the drug target, which involves In Silico drug design to control Rainbow trout fry syndrome. |
