Fine-Mapping and Identification of a Candidate Gene Underlying thed2Dwarfing Phenotype in Pearl Millet,Cenchrus americanus(L.) Morrone
| Autor: | Xiaomei Wu, Nethra Nagarajappa, Sebastien Faure, Katrien M. Devos, Ana Clara Pontaroli, Jeffrey L. Bennetzen, Vinod Jakkula, Francis K Padi, Rajiv K. Parvathaneni |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Genetic Markers
COMPARATIVE GENOMICS Germplasm Chromosomes Artificial Bacterial Pennisetum Candidate gene Molecular Sequence Data Biotecnología Agropecuaria Genes Recessive comparative genomics Investigations P-glycoprotein Genes Plant Ética relacionada con Biotecnología Agrícola Inbred strain Gene mapping Gene Expression Regulation Plant Botany Genetics ATP Binding Cassette Transporter Subfamily B Member 1 Molecular Biology Crosses Genetic Genetics (clinical) Plant Proteins FINE-MAPPING Recombination Genetic Indoleacetic Acids biology HAPLOTYPE ANALYSIS Chromosome Mapping P-GLYCOPROTEIN purl.org/becyt/ford/4.4 [https] food and beverages Biological Transport Molecular Sequence Annotation Sorghum biology.organism_classification Dwarfing Phenotype Haplotypes fine-mapping Genetic Loci CIENCIAS AGRÍCOLAS Genetic marker haplotype analysis Mutation purl.org/becyt/ford/4 [https] |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| DOI: | 10.1534/g3.113.005587 |
| Popis: | Pearl millet is one of the most important subsistence crops grown in India and sub-Saharan Africa. In many cereal crops, reduced height is a key trait for enhancing yield, and dwarf mutants have been extensively used in breeding to reduce yield loss due to lodging under intense management. In pearl millet, the recessive d2 dwarfing gene has been deployed widely in commercial germplasm grown in India, the United States, and Australia. Despite its importance, very little research has gone into determining the identity of the d2 gene. We used comparative information, genetic mapping in two F2 populations representing a total of some 1500 progeny, and haplotype analysis of three tall and three dwarf inbred lines to delineate the d2 region by two genetic markers that, in sorghum, define a region of 410 kb with 40 annotated genes. One of the sorghum genes annotated within this region is ABCB1, which encodes a P-glycoprotein involved in auxin transport. This gene had previously been shown to underlie the economically important dw3 dwarf mutation in sorghum. The cosegregation of ABCB1 with the d2 phenotype, its differential expression in the tall inbred ICMP 451 and the dwarf inbred Tift 23DB, and the similar phenotype of stacked lower internodes in the sorghum dw3 and pearl millet d2 mutants suggest that ABCB1 is a likely candidate for d2. Fil: Parvathaneni, Rajik. University of Georgia; Estados Unidos Fil: Jakkula, Vinod. University of Georgia; Estados Unidos Fil: Padi, Francis. Cocoa Research Institute of Ghana; Ghana Fil: Faure, Sebastien. Cereals Genetics and Genomics; Francia Fil: Nagarajappa, Nethra. University of Georgia; Estados Unidos Fil: Pontaroli, Ana. University of Georgia; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Wu, Xiaome. University of Georgia; Estados Unidos Fil: Bennetzen, Jeffrey. University of Georgia; Estados Unidos Fil: Devos, Katriem. University of Georgia; Estados Unidos |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|