The presence of a 5′-abasic lesion enhances discrimination of single nucleotide polymorphisms while inducing an isothermal ligase chain reaction
| Autor: | Eiman A. Osman, Julianne M. Gibbs-Davis, Tendai Gadzikwa, Abu Kausar |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification DNA ligase Single-nucleotide polymorphism 010402 general chemistry Dna amplification 01 natural sciences Biochemistry Molecular biology 0104 chemical sciences Analytical Chemistry Lesion 03 medical and health sciences 030104 developmental biology chemistry Electrochemistry medicine Environmental Chemistry medicine.symptom Ligation Ligase chain reaction Spectroscopy |
| Zdroj: | The Analyst. 141:4272-4277 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c6an00614k |
| Popis: | Lesion-induced DNA amplification (LIDA) has been employed in the detection of single nucleotide polymorphisms (SNPs). Due to the presence of the proximal abasic lesion, T4 DNA ligase exhibits greater intolerance to basepair mismatches when compared with mismatch ligation in the absence of the abasic lesion. Moreover the presence of the abasic group also results in an isothermal ligase chain reaction enabling SNP detection with great discrimination and sensitivity. Specifically, at forty minutes, the ratio of amplified product from the matched and mismatched initiated reactions are 7-12 depending on the mismatch. The ease of implementation of our method is demonstrated by real-time analysis of DNA amplification using a fluorescent plate reader. |
| Databáze: | OpenAIRE |
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