Feasibility of iFISH patterns in hematologic malignancies among Congolese patients at Kinshasa University clinics
| Autor: | Jacques Bikaula Ngwidiwo, Mbombo Aurore Cecilia Orphée Beia, Kuanda Thomas Solo, Benjamin Longo-Mbenza, Mireille Solange Nganga Nkanga, Venant Tchokonte-Nana, Antoine Lufimbo Katawandja, Fons Verdonck, Ntolo Jean-Pierre Mufuta, Nkama Claude Nlandu, Peter Vandenberghe, Paul Roger Beia Kazadi, Roth Laure Mapapa Miakassissa |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Pediatrics lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences 0302 clinical medicine hemic and lymphatic diseases Internal medicine medicine lcsh:QH301-705.5 medicine.diagnostic_test business.industry Myeloid leukemia Remote analysis medicine.disease Peripheral blood Leukemia 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) 030220 oncology & carcinogenesis Bone marrow business Fluorescence in situ hybridization |
| Zdroj: | Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 12, Pp 1116-1119 (2017) |
| ISSN: | 2221-1691 |
| Popis: | Objective: To analyze the feasibility of detecting Ph1 in leukemia patients in the Kinshasa University Clinics in the Democratic Republic of Congo, at KU Leuven, Belgium. Methods: Bone marrow and peripheral blood samples with chronic myeloid leukemia, acute myeloid leukemia or acute leukocytes leukemia were obtained from 32 patients in Kinshasa University clinics in the Democratic Republic of Congo and transferred to KU Leuven in Belgium for iFISH feasibility. Ph1 was detected by using a remote analysis of interphase fluorescence in situ hybridization (iFISH). Results: Out of the 32 patients involved in this study, 65.6% (n = 21) of the cases were successfully tested, of which 52.4% (n = 11) were iFISH positives for the variant t(9;22) (presence of Ph1) in chronic myeloid leukemia samples and 47.6% (n = 10) negatives in all subtypes of hematological malignancies. However, there was a female predominance in chronic myeloid leukemia samples Ph1-positives by iFISH, whereas no sexual influence was observed on acute subtypes of leukemia. Conclusions: iFISH analysis is feasible on samples obtained from remote sites in the Democratic Republic of Congo. However, the optimization of the sample storage is necessary to further improve iFISH's performance. Keywords: iFISH, Ph1, Democratic Republic of Congo, Leukemia, Bone marrow, Blood |
| Databáze: | OpenAIRE |
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