Molecular Response of Crop Plants to Engineered Nanomaterials
| Autor: | Marta Marmiroli, Joseph Hawthorne, Om Parkash Dhankher, Roberto De La Torre-Roche, Arnab Mukherjee, Jason C. White, Susan M. Isch, Robert E. Marra, Elena Maestri, Sanghamitra Majumdar, Alia D. Servin, Nelson Marmiroli, Luca Pagano |
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| Rok vydání: | 2016 |
| Předmět: |
DNA repair
Arabidopsis 02 engineering and technology Computational biology 010501 environmental sciences 01 natural sciences Cucurbita pepo Solanum lycopersicum Gene Expression Regulation Plant Gene expression Botany Environmental Chemistry Arabidopsis thaliana Gene 0105 earth and related environmental sciences Regulation of gene expression biology fungi food and beverages General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification Nanostructures Gene Ontology Molecular Response Solanum 0210 nano-technology |
| Zdroj: | Environmental sciencetechnology. 50(13) |
| ISSN: | 1520-5851 |
| Popis: | Functional toxicology has enabled the identification of genes involved in conferring tolerance and sensitivity to engineered nanomaterial (ENM) exposure in the model plant Arabidopsis thaliana (L.) Heynh. Several genes were found to be involved in metabolic functions, stress response, transport, protein synthesis, and DNA repair. Consequently, analysis of physiological parameters, metal content (through ICP-MS quantification), and gene expression (by RT-qPCR) of A. thaliana orthologue genes were performed across different plant species of agronomic interest to highlight putative biomarkers of exposure and effect related to ENMs. This approach led to the identification of molecular markers in Solanum lycopersicum L. and Cucurbita pepo L. (tomato and zucchini) that might not only indicate exposure to ENMs (CuO, CeO2, and La2O3) but also provide mechanistic insight into response to these materials. Through Gene Ontology (GO) analysis, the target genes were mapped in complex interatomic networks representing molecular pathways, cellular components, and biological processes involved in ENM response. The transcriptional response of 38 (out of 204) candidate genes studied varied according to particle type, size, and plant species. Importantly, some of the genes studied showed potential as biomarkers of ENM exposure and effect and may be useful for risk assessment in foods and in the environment. |
| Databáze: | OpenAIRE |
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