Novaluron impairs the silk gland and productive performance of silkworm Bombyx mori (Lepidoptera: Bombycidae) larvae
| Autor: | Rose Meire Costa, Marilucia Santorum, Daniela Carvalho dos Santos, Gustavo Henrique dos Reis |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), State University of Western Paraná (UNIOESTE), Universidade Federal do Rio de Janeiro (UFRJ) |
| Rok vydání: | 2020 |
| Předmět: |
Morphology
Insecticides Environmental Engineering Health Toxicology and Mutagenesis media_common.quotation_subject 0208 environmental biotechnology Silk 02 engineering and technology Insect 010501 environmental sciences 01 natural sciences Lepidoptera genitalia Bombycidae chemistry.chemical_compound Bombyx mori Insect growth regulator Animals Environmental Chemistry Sericulture Insecticide 0105 earth and related environmental sciences media_common Novaluron Toxicity biology Phenylurea Compounds fungi Public Health Environmental and Occupational Health Epithelial Cells General Medicine General Chemistry Bombyx biology.organism_classification Pollution 020801 environmental engineering Cell biology Lepidoptera chemistry Silk cocoons Ultrastructure Larva |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0045-6535 |
| Popis: | Made available in DSpace on 2020-12-12T01:39:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 This study investigates the effects of the insect growth regulator Novaluron on the silk gland (SG) and silk cocoon production in a nontarget insect, the silkworm Bombyx mori, which is a model research insect among Lepidoptera and of great economic importance for the commercial production of silk threads. Larvae were segregated into experimental groups: the control group (CG) and the treatment group (TG), which was exposed to a Novaluron concentration of 0.15 mL/L. Following exposure, we analyzed the cytotoxic effects on the epithelial cells of the anterior, middle and posterior regions of the SG of B. mori larvae in the 3rd, 4th, and 5th instars, as well as the quality of the cocoons from larvae in the 5th instar. Cytotoxic effects were observed in the TG, such as the dilation of cells, emission of cytoplasmic protrusions, extreme rarefaction of the cytoplasm and nuclei, dilation of the endoplasmic reticulum, intracellular and intercellular spaces, spacing between the epithelial cells and the basal lamina and detachment of some cells towards the lumen of the SG, and decreased protein in the lumen, with faults in its composition. In addition, we verified ultrastructural changes in the production of fibers and silk cocoons, including a reduction in the weight of the cocoons constructed by both males and females in the TG and the construction of defective cocoons. Novaluron exposure impairs the SG and may affect the physiological functions of this organ; additionally, it compromises the quality of silk cocoons, potentially causing serious damage to sericulture. Laboratory of Insects Department of Morphology São Paulo State University (UNESP) Institute of Biosciences Center of Biological Sciences and Health State University of Western Paraná (UNIOESTE) Postgraduate in Pathology Federal University of Rio de Janeiro (UFRJ) Electron Microscopy Center of the Institute of Biosciences of Botucatu UNESP Laboratory of Insects Department of Morphology São Paulo State University (UNESP) Institute of Biosciences Electron Microscopy Center of the Institute of Biosciences of Botucatu UNESP |
| Databáze: | OpenAIRE |
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