Epimorphin Mediates Mammary Luminal Morphogenesis through Control of C/EBPβ
| Autor: | Kyoko Takebe, Derek C. Radisky, Shin-ichiro Niwa, Mina J. Bissell, Rosanne Boudreau, Yohei Hirai, Marina Simian, Yumiko Oka, Mary E. Stevens |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Cell signaling
Morphogenesis Mice Transgenic Cell Communication Biology transgenic mice Mice Mammary Glands Animal Enhancer binding parasitic diseases medicine transcription factor balance Animals Lactation CCAAT/enhancer binding protein Fibroblast Transcription factor Membrane Glycoproteins Ccaat-enhancer-binding proteins Myoepithelial cell Epithelial Cells Cell Biology Milk Proteins Molecular biology medicine.anatomical_structure epithelial–stromal interactions CCAAT-Enhancer-Binding Proteins mammary morphogenesis Original Article Female Stromal Cells Morphogen |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | We have shown previously that epimorphin (EPM), a protein expressed on the surface of myoepithelial and fibroblast cells of the mammary gland, acts as a multifunctional morphogen of mammary epithelial cells. Here, we present the molecular mechanism by which EPM mediates luminal morphogenesis. Treatment of cells with EPM to induce lumen formation greatly increases the overall expression of transcription factor CCAAT/enhancer binding protein (C/EBP)β and alters the relative expression of its two principal isoforms, LIP and LAP. These alterations were shown to be essential for the morphogenetic activities, since constitutive expression of LIP was sufficient to produce lumen formation, whereas constitutive expression of LAP blocked EPM-mediated luminal morphogenesis. Furthermore, in a transgenic mouse model in which EPM expression was expressed in an apolar fashion on the surface of mammary epithelial cells, we found increased expression of C/EBPβ, increased relative expression of LIP to LAP, and enlarged ductal lumina. Together, our studies demonstrate a role for EPM in luminal morphogenesis through control of C/EBPβ expression. |
| Databáze: | OpenAIRE |
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