Analysis of the CD4+ T cell responses to house dust mite allergoid

Autor: M C Lebre, J. W. P. M. van Schijndel, E C de Jong, Martien L. Kapsenberg, P. Kalinski, D. Kramer
Přispěvatelé: Clinical Immunology and Rheumatology, Public and occupational health, Cell Biology and Histology, Amsterdam institute for Infection and Immunity
Rok vydání: 2003
Předmět:
Zdroj: Allergy, 58(7), 648-656. Wiley-Blackwell
ISSN: 0105-4538
Popis: Background: Modified allergen extracts (allergoids) with reduced IgE-binding capacity are successfully used in immunotherapy of atopic allergy. Their reduced T-cell stimulatory capacity is less well studied and is a subject of the present study. Methods: We compared the ability of native house dust mite extract (Dermatophagoides pteronyssinus; HDM) and the glutaraldehyde-modified allergoid (HDM-GA) to induce the proliferation and cytokine production by fresh PBMC and by DC-stimulated polyclonal Th cells and HDM-specific Th cell clones. Results: Freshly isolated T cells showed a partially reduced responsiveness to HDM-GA, differentially pronounced in different donors. HDM-specific Th cell clones prepared from three donors showed either a complete loss of reactivity to HDM-GA, or completely preserved responsiveness. The frequency of nonreactive clones was donor-dependent (2/3, 3/10 and 1/10). GA modification of HDM did not interfere with the cytokine production profile of HDM-specific T cell clones. Conclusions: The reduced stimulatory potential of HDM-GA results mainly from a loss of certain Th cell epitopes, rather than impaired allergen uptake and presentation, or induction of suppressive factors. Varying frequencies of allergoid-nonreactive HDM-specific Th cells may result in differential responses of individual patients to immunotherapy.
Databáze: OpenAIRE