High Intensity Interval Exercise Increases Platelet and Transforming Growth Factor‐β Yield in Platelet‐Rich Plasma

Autor: Michael R. Baria, James Borchers, Robert A. Magnussen, James A. Onate, William Kelton Vasileff, Sushmitha S. Durgam, David C. Flanigan, Meghan M. Miller, Shannon Desmond, Christopher C. Kaeding
Rok vydání: 2020
Předmět:
Blood Platelets
Male
Vascular Endothelial Growth Factor A
030506 rehabilitation
medicine.medical_specialty
medicine.medical_treatment
Pilot Projects
Physical Therapy
Sports Therapy and Rehabilitation
High-Intensity Interval Training
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Somatomedins
Transforming Growth Factor beta
Internal medicine
Humans
Medicine
Platelet
Mean platelet volume
Whole blood
Platelet-Derived Growth Factor
biology
Platelet-Rich Plasma
business.industry
Growth factor
Rehabilitation
Vascular endothelial growth factor
Endocrinology
Neurology
chemistry
Transforming Growth Factors
Platelet-rich plasma
biology.protein
Female
Neurology (clinical)
0305 other medical science
business
030217 neurology & neurosurgery
Platelet-derived growth factor receptor
Transforming growth factor
Zdroj: PM&R. 12:1244-1250
ISSN: 1934-1563
1934-1482
Popis: BACKGROUND Platelet-rich plasma (PRP) is an emerging orthobiologic treatment for musculoskeletal conditions like osteoarthritis. Two studies have demonstrated the influence of longer duration exercise on PRP composition, but no study has ever explored the impact of high intensity interval exercise (HIIE) on PRP content. OBJECTIVE To quantify cellular and growth factor content changes in PRP after 4 minutes of HIIE. DESIGN Controlled laboratory pilot study. SETTING Academic sports medicine center. PARTICIPANTS Ten healthy volunteers (5 male, 5 female). INTERVENTION Volunteers had PRP prepared from 15 mL of whole blood using a single spin, plasma-based system (autologous conditioned plasma [ACP]) immediately before and after 4 minutes of HIIE on a stationary exercise bike (Tabata protocol). MAIN OUTCOME MEASURE The PRP was sent for complete blood counts and enzyme-linked immunosorbent assay (ELISA) to quantify transforming growth factor (TGF)-β, platelet-derived growth factor (PDGF), insulin-like growth factor (IGF)-1, and vascular endothelial growth factor (VEGF). RESULTS Mean platelet count in PRP increased from 367.4 ± 57.5 k/μL to 497.7 ± 93.3 k/μL after 4 minutes of HIIE (P
Databáze: OpenAIRE
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