High Intensity Interval Exercise Increases Platelet and Transforming Growth Factor‐β Yield in Platelet‐Rich Plasma
Autor: | Michael R. Baria, James Borchers, Robert A. Magnussen, James A. Onate, William Kelton Vasileff, Sushmitha S. Durgam, David C. Flanigan, Meghan M. Miller, Shannon Desmond, Christopher C. Kaeding |
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Rok vydání: | 2020 |
Předmět: |
Blood Platelets
Male Vascular Endothelial Growth Factor A 030506 rehabilitation medicine.medical_specialty medicine.medical_treatment Pilot Projects Physical Therapy Sports Therapy and Rehabilitation High-Intensity Interval Training 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Somatomedins Transforming Growth Factor beta Internal medicine Humans Medicine Platelet Mean platelet volume Whole blood Platelet-Derived Growth Factor biology Platelet-Rich Plasma business.industry Growth factor Rehabilitation Vascular endothelial growth factor Endocrinology Neurology chemistry Transforming Growth Factors Platelet-rich plasma biology.protein Female Neurology (clinical) 0305 other medical science business 030217 neurology & neurosurgery Platelet-derived growth factor receptor Transforming growth factor |
Zdroj: | PM&R. 12:1244-1250 |
ISSN: | 1934-1563 1934-1482 |
Popis: | BACKGROUND Platelet-rich plasma (PRP) is an emerging orthobiologic treatment for musculoskeletal conditions like osteoarthritis. Two studies have demonstrated the influence of longer duration exercise on PRP composition, but no study has ever explored the impact of high intensity interval exercise (HIIE) on PRP content. OBJECTIVE To quantify cellular and growth factor content changes in PRP after 4 minutes of HIIE. DESIGN Controlled laboratory pilot study. SETTING Academic sports medicine center. PARTICIPANTS Ten healthy volunteers (5 male, 5 female). INTERVENTION Volunteers had PRP prepared from 15 mL of whole blood using a single spin, plasma-based system (autologous conditioned plasma [ACP]) immediately before and after 4 minutes of HIIE on a stationary exercise bike (Tabata protocol). MAIN OUTCOME MEASURE The PRP was sent for complete blood counts and enzyme-linked immunosorbent assay (ELISA) to quantify transforming growth factor (TGF)-β, platelet-derived growth factor (PDGF), insulin-like growth factor (IGF)-1, and vascular endothelial growth factor (VEGF). RESULTS Mean platelet count in PRP increased from 367.4 ± 57.5 k/μL to 497.7 ± 93.3 k/μL after 4 minutes of HIIE (P |
Databáze: | OpenAIRE |
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