Real-time pathogen monitoring during enrichment: a novel nanotechnology-based approach to food safety testing
| Autor: | Kristin Weidemaier, Mark Muldoon, Douglas B. Sherman, Adam C. Curry, Melody Kuroda, Joseph Thomas, Eric Fallows, Erin Carruthers |
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| Rok vydání: | 2014 |
| Předmět: |
Salmonella
Food Safety Meat Listeria Nanotechnology medicine.disease_cause Microbiology food medicine Escherichia coli Food microbiology Animals Sample preparation Food science Orange juice Immunoassay medicine.diagnostic_test biology business.industry General Medicine biology.organism_classification Food safety food.food Chocolate milk Food Microbiology Environmental science Dairy Products business Food Science |
| Zdroj: | International journal of food microbiology. 198 |
| ISSN: | 1879-3460 |
| Popis: | We describe a new approach for the real-time detection and identification of pathogens in food and environmental samples undergoing culture. Surface Enhanced Raman Scattering (SERS) nanoparticles are combined with a novel homogeneous immunoassay to allow sensitive detection of pathogens in complex samples such as stomached food without the need for wash steps or extensive sample preparation. SERS-labeled immunoassay reagents are present in the cultural enrichment vessel, and the signal is monitored real-time through the wall of the vessel while culture is ongoing. This continuous monitoring of pathogen load throughout the enrichment process enables rapid, hands-free detection of food pathogens. Furthermore, the integration of the food pathogen immunoassay directly into the enrichment vessel enables fully biocontained food safety testing, thereby significantly reducing the risk of contaminating the surrounding environment with enriched pathogens. Here, we present experimental results showing the detection of E. coli, Salmonella, or Listeria in several matrices (raw ground beef, raw ground poultry, chocolate milk, tuna salad, spinach, brie cheese, hot dogs, deli turkey, orange juice, cola, and swabs and sponges used to sample a stainless steel surface) using the SERS system and demonstrate the accuracy of the approach compared to plating results. |
| Databáze: | OpenAIRE |
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