Complete nucleotide sequence and biological properties of an infectious clone of prototype echovirus 9
| Autor: | Werner Kraus, Hans J. Eggers, Holger Zimmermann, Albert Zimmermann, Birgit Nelsen-Salz |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Cancer Research
Echovirus viruses Molecular Sequence Data Biology Recombinant virus medicine.disease_cause law.invention Mice Capsid law Virology medicine Animals Humans Amino Acid Sequence Echovirus 9 Base Sequence Nucleic acid sequence virus diseases RNA Molecular biology Disease Models Animal Open reading frame Infectious Diseases Protein Biosynthesis Recombinant DNA RNA Viral Protein Processing Post-Translational |
| Zdroj: | Virus Research. 39:311-319 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/0168-1702(95)00078-x |
| Popis: | The prototype Hill of echovirus 9, a human enterovirus, exhibits no pathogenicity for newborn mice in contrast to some other echovirus 9 strains isolated subsequently during epidemics. In this communication we report the first complete nucleotide sequence and construction of an infectious clone of echovirus 9. Aside from the 3′ poly(A)-tract, the RNA genome is 7420 nucleotides (nt) in length and encodes a single polyprotein of 2193 amino acids (aa). The open reading frame extends from position 740 to position 7318 of the genome. Sequence comparisons to other enteroviruses reveal a strong overall amino acid identity to echovirus types 11 and 12 (in each case 76%). The proteolytic cleavage sites of the three major capsid proteins were determined after purification by HPLC and protein sequencing. A full-length clone coding for an infectious RNA transcript was constructed, and recombinant echovirus 9 particles could be isolated from the supernatant of transfected cell culture. It is shown that the recombinant virus, like the original prototype, is non-pathogenic for newborn mice and does not multiply in skeletal muscles. |
| Databáze: | OpenAIRE |
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