Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure
| Autor: | Eva Václavková, Kristyna Smerkova, Daria Baholet, David Hynek, Zuzana Lackova, Vedran Milosavljevic, Sylvie Skalickova, Pavel Kopel, Pavel Nevrkla, Vojtech Adam, Zuzana Bytesnikova, Jiri Skladanka, Pavel Knot, Lenka Urbankova, Eliska Kabourkova, Silvia Kociova, Milica Gagic, Vendula Smolikova, Pavel Horky, Olga Krystofova, Natalia Cernei |
|---|---|
| Přispěvatelé: | Analytical, Environmental & Geo-Chemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Antioxidant Coliforms Aerobic bacteria medicine.drug_class medicine.medical_treatment Antibiotics chemistry.chemical_element Zinc medicine.disease_cause Biochemistry 03 medical and health sciences In vivo medicine Food science lcsh:SF1-1100 Antibacterial agent Nanomaterials lcsh:Veterinary medicine Chemistry Research 0402 animal and dairy science 04 agricultural and veterinary sciences Antimicrobial 040201 dairy & animal science 030104 developmental biology Staphylococcus aureus Oxidative stress lcsh:SF600-1100 Animal Science and Zoology lcsh:Animal culture Food Science Biotechnology |
| Zdroj: | Journal of Animal Science and Biotechnology. 2019, vol. 10, issue 1, p. 1-12. Journal of Animal Science and Biotechnology Journal of Animal Science and Biotechnology, Vol 10, Iss 1, Pp 1-12 (2019) |
| Popis: | Background Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens. Results Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest in vitro inhibitory effect of our NPs was observed against Staphylococcus aureus (inhibitory concentration values, IC50, ranged from 0.5 to 1.6 mmol/L), followed by Escherichia coli (IC50 0.8–1.5 mmol/L). In contrast, methicillin resistant S. aureus (IC50 1.2–4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful in vitro testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|