Retention of the herpes simplex virus type 1 (HSV-1) UL37 protein on single-stranded DNA columns requires the HSV-1 ICP8 protein
| Autor: | W T Ruyechan, F. J. Jenkins, A. G. Albright, L. S. G. Shelton |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
ICP8
viruses Immunology DNA Single-Stranded medicine.disease_cause Microbiology DNA-binding protein Chromatography Affinity Virus Herpesviridae law.invention Viral Proteins chemistry.chemical_compound law Virology Protein A/G medicine Simplexvirus Viral Structural Proteins biology biochemical phenomena metabolism and nutrition Molecular biology Recombinant Proteins DNA-Binding Proteins Herpes simplex virus chemistry Insect Science DNA Viral biology.protein Recombinant DNA Vaccinia Research Article |
| Zdroj: | Journal of Virology. 68:521-525 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.68.1.521-525.1994 |
| Popis: | The UL37 and ICP8 proteins present in herpes simplex virus type 1 (HSV-1)-infected-cell extracts produced at 24 h postinfection coeluted from single-stranded-DNA-cellulose columns. Experiments carried out with the UL37 protein expressed by a vaccinia virus recombinant (V37) revealed that the UL37 protein did not exhibit DNA-binding activity in the absence of other HSV proteins. Analysis of extracts derived from cells coinfected with V37 and an ICP8-expressing vaccinia virus recombinant (V8) and analysis of extracts prepared from cells infected with the HSV-1 ICP8 deletion mutants d21 and n10 revealed that the retention of the UL37 protein on single-stranded DNA columns required a DNA-binding-competent ICP8 protein. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|