Sequencing and analysis of the complete mitochondrial genome of Ochotona hyperborea from China and its phylogenetic analysis
| Autor: | Jun-Sheng Zhang, Zhong-Wan Piao, Zhu Liu, Qi Zhang, Xin-Xu Zhao, Qian-Qian Yao, Qiao-Jiang Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics Mitochondrial DNA biology Phylogenetic tree mitogenome Long pcr Coreana biology.organism_classification 010603 evolutionary biology 01 natural sciences Genome phylogenetic trees 03 medical and health sciences Ochotona hyperborea 030104 developmental biology Control region Transfer RNA Molecular Biology Gene Mitogenome Announcement Research Article |
| Zdroj: | Mitochondrial DNA. Part B, Resources article-version (VoR) Version of Record |
| ISSN: | 2380-2359 |
| Popis: | The complete mitogenome sequence of Ochotona hyperborea was determined using long PCR. The genome was 17,063 bp in length and contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, one origin of L strand replication, and one control region. The overall base composition of the heavy strand is A (31.1%), C (28.7%), T (26.3%), and G (13.9%). The base compositions present clearly the A–T skew, which is most obvious in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ, and Bayesian analyses yielded identical phylogenetic trees. This study verifies the evolutionary status of Ochotona hyperborea in Ochotonidae at the molecular level. The mitochondrial genome would be a significant supplement for the Ochotona hyperborea genetic background. The eight Ochotona species formed a monophyletic group with the high bootstrap value (100%) in all examinations. |
| Databáze: | OpenAIRE |
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