Elm tree bark extract inhibits HepG2 hepatic cancer cell growth via pro-apoptotic activity
| Autor: | So Young Youm, Tae Myoung Kim, Sang Kyung Shin, Dae Joong Kim, Tae Wang Kim, Byeongwoo Ahn |
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| Rok vydání: | 2012 |
| Předmět: |
Carcinoma
Hepatocellular Indoles Cell Survival Ulmus Blotting Western HepG2 cell medicine.disease_cause chemistry.chemical_compound Bcl-2-associated X protein medicine Humans Viability assay bcl-2-Associated X Protein General Veterinary biology Caspase 3 Plant Extracts Ulmus davidiana Liver Neoplasms apoptosis Cancer elm tree extract Hep G2 Cells Flow Cytometry medicine.disease Caspase 9 Biochemistry chemistry Apoptosis Cancer cell Plant Bark Cancer research biology.protein Original Article Poly(ADP-ribose) Polymerases Growth inhibition Liver cancer Carcinogenesis |
| Zdroj: | Journal of Veterinary Science |
| ISSN: | 1976-555X 1229-845X |
| Popis: | Control of inflammation is widely accepted as an important strategy for cancer chemoprevention. Anti-inflammatory effects of bark extracts of elm tree (BEE) have been amply reported. Therefore, BEE may be a good candidate cancer chemopreventive agent. Considering the high incidence of hepatic cancer and limited therapeutic approaches for treating this disease, it is important to develop liver cancer-specific chemopreventive agents. To evaluate the chemopreventive potential of BEE, we investigated the growth inhibition effect of BEE on the HepG2 human hepatocellular carcinoma cell line. We performed a cell counting kit-8 assay to determine cell viability, and 4,6-diamino-2-phenylindole staining and flow cytometry to measure apoptotic cell death. Finally, the expression levels of pro- and anti-apoptotic proteins were measured. BEE inhibited the growth of HepG2 cells and induced apoptosis in a dose-dependent manner. Pro-apoptotic activity was promoted via the mitochondrial pathway of apoptosis, as demonstrated by the activation of pro-apoptotic proteins Bax, caspase-9, caspase-3, and poly (ADP-ribose) polymerase as well as the down-regulation of the anti-apoptotic protein Bcl-2. These results suggest that BEE may have potential use in hepatic cancer chemoprevention by suppressing cancer cell growth via pro-apoptotic activity. |
| Databáze: | OpenAIRE |
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