Expression analysis of VEGF-A and VEGF-B: relationship with clinicopathological parameters in bladder cancer
| Autor: | Franck Monnien, Guillaume Boiteux, Hugues Bittard, Isabelle Lascombe, Sylvie Fauconnet, Anne Clairotte, Stéphane Bernardini, Eric Chabannes |
|---|---|
| Přispěvatelé: | Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC ( CEF2P / CARCINO ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC ), Service de Néphrologie et Urologie, Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Hôpital Saint-Jacques, Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC (EA 3181) (CEF2P / CARCINO), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Service d'urologie, andrologie et transplantation rénale, Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Hôpital Saint-Jacques |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Vascular Endothelial Growth Factor A
Cancer Research Pathology Vascular Endothelial Growth Factor B Time Factors MESH : RNA Messenger MESH : Alternative Splicing Kaplan-Meier Estimate urologic and male genital diseases MESH : Neoplasm Invasiveness [ SDV.CAN ] Life Sciences [q-bio]/Cancer 0302 clinical medicine MESH: Reverse Transcriptase Polymerase Chain Reaction MESH : Tumor Markers Biological MESH: Up-Regulation MESH: Blotting Southern MESH : Neoplasm Staging MESH: Kaplan-Meiers Estimate MESH : Up-Regulation MESH: Treatment Outcome 0303 health sciences Reverse Transcriptase Polymerase Chain Reaction MESH: Alternative Splicing MESH : Blotting Northern MESH : Reverse Transcriptase Polymerase Chain Reaction General Medicine MESH: Neoplasm Staging MESH: Gene Expression Regulation Neoplastic MESH: Urinary Bladder Neoplasms Up-Regulation Blot Gene Expression Regulation Neoplastic Blotting Southern Treatment Outcome MESH : Carcinoma in Situ Oncology MESH : Urinary Bladder Neoplasms 030220 oncology & carcinogenesis MESH : Vascular Endothelial Growth Factor B MESH : Vascular Endothelial Growth Factor A MESH : Disease-Free Survival Carcinoma in Situ MESH : Time Factors medicine.medical_specialty MESH: Cell Line Tumor MESH : Gene Expression Regulation Neoplastic [SDV.CAN]Life Sciences [q-bio]/Cancer MESH : Treatment Outcome Disease-Free Survival Andrology 03 medical and health sciences Cell Line Tumor medicine Carcinoma Biomarkers Tumor MESH: Blotting Northern Humans Neoplasm Invasiveness Northern blot RNA Messenger Urothelium 030304 developmental biology MESH: RNA Messenger Neoplasm Staging MESH: Carcinoma in Situ MESH: Carcinoma Transitional Cell Carcinoma Transitional Cell Bladder cancer MESH: Humans MESH : Carcinoma Transitional Cell Oncogene business.industry MESH : Cell Line Tumor Carcinoma in situ MESH: Vascular Endothelial Growth Factor A MESH: Time Factors MESH: Vascular Endothelial Growth Factor B MESH : Humans Cancer MESH : Kaplan-Meiers Estimate MESH: Neoplasm Invasiveness MESH : Blotting Southern medicine.disease Blotting Northern Alternative Splicing Urinary Bladder Neoplasms MESH: Tumor Markers Biological MESH: Disease-Free Survival business |
| Zdroj: | Oncology Reports Oncology Reports, Spandidos Publications, 2009, 21 (6), pp.1495-504 |
| ISSN: | 1021-335X |
| Popis: | International audience; The present investigation was conducted first to determine whether correlation exists between VEGF-A and -B mRNA levels and clinicopathological parameters and to assess their prognostic value in bladder cancer, then to clarify the expression level and biological significance of VEGF-A isoforms. Total RNA was isolated from 37 specimens of bladder cancer. Northern blot analysis revealed that VEGF-B mRNA was not expressed either in normal urothelium or in bladder cancer and detected three VEGF-A transcripts of 5.2, 4.5 and 1.7 kb in length, respectively. The VEGF-A transcript levels were greater in cancer tissues than in normal urothelium. They were significantly higher in pT2-T4 than in pTa and pT1 urothelial tumors and thus, were correlated to the pathologic stage. Contrary to the 4.5 kb transcript, elevated expression of the 5.2 and 1.7 kb transcripts was correlated with the histologic grade and the presence of carcinoma in situ. Patients with higher VEGF-A mRNA levels had a significantly shorter survival without progression compared to those with lower levels. Three VEGF-A splice variants were detected by southern blotting namely, VEGF121, 165 and 189. The expression intensity of each isoform was evaluated by quantitative real-time RT-PCR in 20 new fresh frozen recent tumors. VEGF121 and VEGF165 were expressed at the similar level. On the contrary, they were significantly more expressed than VEGF189 (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|