Control of Sister Chromatid Recombination by Histone H2AX
| Autor: | Nadine Puget, Craig H. Bassing, Inbo Shim, Ralph Scully, Shobu Odate, Anyong Xie, Frederick W. Alt, Ingeborga Jarzyna |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Genome instability
cells RAD51 Chromatids Biology environment and public health Article Histones Homology directed repair Mice Serine Animals Sister chromatids Molecular Biology Recombination Genetic Genetics BRCA1 Protein Cell Biology Chromatin DNA-Binding Proteins enzymes and coenzymes (carbohydrates) Histone biology.protein Chromatid Rad51 Recombinase biological phenomena cell phenomena and immunity Homologous recombination |
| Zdroj: | Molecular Cell. 16:1017-1025 |
| ISSN: | 1097-2765 |
| Popis: | Histone H2AX has a role in suppressing genomic instability and cancer. However, the mechanisms by which it performs these functions are poorly understood. After DNA breakage, H2AX is phosphorylated on serine 139 in chromatin near the break. We show here that H2AX serine 139 enforces efficient homologous recombinational repair of a chromosomal double-strand break (DSB) by using the sister chromatid as a template. BRCA1, Rad51, and CHK2 contribute to recombinational repair, in part independently of H2AX. H2AX(-/-) cells show increased use of single-strand annealing, an error-prone deletional mechanism of DSB repair. Therefore, the chromatin response around a chromosomal DSB, in which H2AX serine 139 phosphorylation plays a central role, "shapes" the repair process in favor of potentially error-free interchromatid homologous recombination at the expense of error-prone repair. H2AX phosphorylation may help set up a favorable disposition between sister chromatids. |
| Databáze: | OpenAIRE |
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