Identification of specific and common diagnostic antibody markers for gastrointestinal cancers by SEREX screening using testis cDNA phage library
| Autor: | Hideo Shin, Masaaki Ito, Kouichi Kitamura, Fumio Nomura, Masaki Takiguchi, Yuji Sawabe, Masae Suzuki, Sohei Kobayashi, Hideaki Shimada, Takaki Hiwasa, Akiko Kagaya, Masayuki Kano, Takahiro Arasawa, Sayaka Ishii, Hisahiro Matsubara, Bahityar Rahmutulla, Kazuyuki Matsushita |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Colorectal cancer 03 medical and health sciences 0302 clinical medicine Antigen medicine gastrointestinal cancers AlphaLISA biology business.industry Cancer SEREX medicine.disease Molecular biology esophageal squamous cell carcinoma HOOK2 Blot 030104 developmental biology Oncology 030220 oncology & carcinogenesis biology.protein Cancer/testis antigens BAMBI Antibody cancer-testis antigen business Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.24963 |
| Popis: | The present study was planned to identify novel serum antibody markers for digestive organ cancers. We have used screening by phage expression cloning and identified novel fourteen antigens in this experiment. The presence of auto-antibodies against these antigens in serum specimens was confirmed by western blotting. As for auto-antibodies against fourteen antigens, AlphaLISA (amplified luminescence proximity homogeneous assay) assay was performed in the sera of gastrointestinal cancers patients to confirm the results. Serum antibody levels against these fourteen recombinant proteins as antigens between healthy donors (HD) and esophageal squamous cell carcinoma (ESCC) patients, gastric cancer (GC), or colon cancer (CC) were compared. The serum levels of all fourteen auto-antibodies were significantly higher in ESCC and GC than those of HD. Among those auto-antibodies, except ECSA2 and CCNL2, were also detected significantly higher levels in CC than those of HD. Receiver operating curve (ROC) revealed similar results except CCNL2 in CC. AUC values calculated by ROC were higher than 0.7 in auto-antibodies against TPI1, HOOK2, PUF60, PRDX4, HS3ST1, TUBA1B, TACSTD2, AKR1C3, BAMBI, DCAF15 in ESCC, auto-antibodies against TPI1, HOOK2, PUF60, PRDX4, TACSTD2, AKR1C3, BAMBI, DCAF15 in GC, and auto-antibodies against TPI1, HOOK2, PUF60 in CC. AUC of the combination of HOOK2 and anti-p53 antibodies in ESCC was observed to be as high as 0.8228. Higher serum antibody levels against ten antigens could be potential diagnostic tool for ESCC. Higher serum antibody levels against eight antigens could be potential diagnostic tool for GC, and serum antibody levels against three antigens could be potential diagnostic tool for CC. |
| Databáze: | OpenAIRE |
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