Use of the dynamic gastro-intestinal model TIM to explore the survival of the yogurt bacterium Streptococcus thermophilus and the metabolic activities induced in the simulated human gut
| Autor: | Yves Le Roux, Emilie Lorson, Stéphanie Blanquet-Diot, Sylvain Denis, Annie Dary, Clarisse Perrin, Ophélie Uriot, Yvonne Roussel, Sandrine Chalancon, Chantal Poirson, Monique Alric, Maira Junjua, Ahoefa Ablavi Awussi, Wessam Galia |
|---|---|
| Přispěvatelé: | Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Conception, Ingénierie et Développement de l'Aliment et du Médicament (CIDAM), Université d'Auvergne - Clermont-Ferrand I (UdA), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Models Anatomic Streptococcus thermophilus Survival [SDV]Life Sciences [q-bio] 030106 microbiology Microbiology law.invention Gastric Acid 03 medical and health sciences Probiotic Upper Gastrointestinal Tract law RIVET (Recombinase In Vivo Expression Technology) Heat shock protein Animals Humans 2. Zero hunger Microbial Viability biology Probiotics TIM (TNO gastrointestinal model) food and beverages biology.organism_classification Yogurt Adaptation Physiological Urease In vitro 030104 developmental biology Milk Biochemistry Gastric acid resistance Genes Bacterial Gastric acid Fermentation Digestion Bacteria Food Science |
| Zdroj: | Food Microbiology Food Microbiology, Elsevier, 2016, 53 (A), pp.18-29. ⟨10.1016/j.fm.2015.05.007⟩ |
| ISSN: | 0740-0020 1095-9998 |
| DOI: | 10.1016/j.fm.2015.05.007⟩ |
| Popis: | 19th Meeting of the Club of Lactic Acid Bacteria, Bordeaux, FRANCE, OCT 16-18, 2013; International audience; Streptococcus thermophilus, a lactic acid bacterium used to produce yogurts and cheeses is more and more considered for its potential probiotic properties. This implies that additional information should be obtained regarding its survival and metabolic activity in the human Gastro-Intestinal Tract (GIT). In this study, we screened 30 S. thermophilus strains for urease, small heat shock protein, and amino-acid decarboxylase functions which may play a role in survival in the upper part of the GIT. The survival kinetics of 4 strains was investigated using the TIM, a physiologically relevant in vitro dynamic gastric and small intestinal model. The three strains LMD9, PB18O and EBLST20 showed significantly higher survival than CNRZ21 in all digestive compartments of the TIM, which may be related to the presence of urease and heat shock protein functions. When LMD9 bacterial cells were delivered in a fermented milk formula, a significant improvement of survival in the TIM was observed compared to non-fermented milk. With the RIVET (Recombinase In Vivo Expression Technology) method applied to the LMD9 strain, a promoter located upstream of hisS, responsible for the histidyl-transfer RNA synthesis, was found to be specifically activated in the artificial stomach. The data generated on S. thermophilus survival and its adaptation capacities to the digestive tract are essential to establish a list of biomarkers useful for the selection of probiotic strains. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect