The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration
| Autor: | Hani Gabra, Carla Burrows, Anne E. Willis, David M. Glover, Sarah P. Blagden, Sarah Jane Lam, George Tzolovsky, Martin Bushell, Kirsty Sawicka, Normala Abd Latip, Lee Carpenter |
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| Přispěvatelé: | Glover, David [0000-0003-0956-0103], Willis, Anne [0000-0002-1470-8531], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
DYNAMICS
Biochemistry & Molecular Biology TRANSLATION INITIATION Cell division INVASION 05 Environmental Sciences Mitosis RNA-binding protein Apoptosis POLY(A)-BINDING PROTEIN Biology Ribosome Autoantigens Poly(A)-Binding Proteins MECHANISMS RNA interference Cell Movement MOTILITY Poly(A)-binding protein Genetics Protein biosynthesis Initiation factor Humans FILOPODIA Pseudopodia Peptide Chain Initiation Translational Science & Technology COMPLEX 06 Biological Sciences Cell cycle Actins Cell biology DROSOPHILA Cytoskeletal Proteins ORTHOLOGUE Eukaryotic Initiation Factor-4E Ribonucleoproteins biology.protein RNA 08 Information and Computing Sciences Life Sciences & Biomedicine Developmental Biology HeLa Cells |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The RNA binding protein Larp1 was originally shown to be involved in spermatogenesis, embryogenesis and cell-cycle progression in Drosophila. Our data show that mammalian Larp1 is found in a complex with poly A binding protein and eukaryote initiation factor 4E and is associated with 60S and 80S ribosomal subunits. A reduction in Larp1 expression by siRNA inhibits global protein synthesis rates and results in mitotic arrest and delayed cell migration. Consistent with these data we show that Larp1 protein is present at the leading edge of migrating cells and interacts directly with cytoskeletal components. Taken together, these data suggest a role for Larp1 in facilitating the synthesis of proteins required for cellular remodelling and migration. |
| Databáze: | OpenAIRE |
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