Evaluation of seasonal influenza vaccines for H1N1pdm09 and type B viruses based on a replication-incompetent PB2-KO virus
| Autor: | Maki Kiso, Kohei Oishi, Hiroki Ui, Yoshihiro Kawaoka, Ryuta Uraki, Shin Murakami, Seiya Yamayoshi, Shigetaka Mimori |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
viruses Respiratory System Hemagglutinin (influenza) Antibodies Viral H5N1 genetic structure Virus Antigenic drift Microbiology 03 medical and health sciences Gene Knockout Techniques Viral Proteins Influenza A Virus H1N1 Subtype Orthomyxoviridae Infections Animals Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology Immunogenicity Public Health Environmental and Occupational Health virus diseases biochemical phenomena metabolism and nutrition Viral Load Virology respiratory tract diseases Immunoglobulin A Vaccination Disease Models Animal Influenza B virus 030104 developmental biology Infectious Diseases Viral replication Influenza Vaccines Immunoglobulin G biology.protein Molecular Medicine Female Neuraminidase |
| Zdroj: | Vaccine. 35(15) |
| ISSN: | 1873-2518 |
| Popis: | Vaccination is the first line of protection against influenza virus infection in humans. Although inactivated and live-attenuated vaccines are available, each vaccine has drawbacks in terms of immunogenicity and safety. To overcome these issues, our group has developed a replication-incompetent PB2-knockout (PB2-KO) influenza virus that replicates only in PB2-expressing cells. Here we generated PB2-KO viruses possessing the hemagglutinin (HA) and neuraminidase (NA) segments from H1N1pdm09 or type B viruses and tested their vaccine potential. The two PB2-KO viruses propagated efficiently in PB2-expressing cells, and expressed chimeric HA as expected. Virus-specific IgG and IgA antibodies were detected in mice immunized with the viruses, and the immunized mice showed milder clinical signs and/or lower virus replication levels in the respiratory tract upon virus challenge. Our results indicate that these PB2-KO viruses have potential as vaccine candidates. |
| Databáze: | OpenAIRE |
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