High throughput method for quantifying androstenone and skatole in adipose tissue from uncastrated male pigs by laser diode thermal desorption-tandem mass spectrometry
| Autor: | Claus Borggaard, Rune Isak Dupont Birkler, Susanne Støier, Birgitte Winther Lund, Kirsten Jensen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male pig odour
Boar taint lcsh:TX341-641 Tandem mass spectrometry Mass spectrometry Article Analytical Chemistry chemistry.chemical_compound Androstenone MS/MS Detection limit Reproducibility Chromatography lcsh:TP368-456 Assay Laser diode thermal desorption Skatole lcsh:Food processing and manufacture chemistry Skatole PubChem CID:6736 Androstenone PubChem CID 6852393 lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Food Chemistry: X, Vol 9, Iss, Pp 100113-(2021) Food Chemistry: X |
| ISSN: | 2590-1575 |
| Popis: | Highlights • Boar taint is mainly caused by the compounds, skatole and androstenone. • Analysis for at-line industrial measurement of boar taint in pigs. • High throughput quantitative analysis of skatole and androstenone. • Low cost, robust and selective analytical results. The study aims at developing a rapid and robust mass spectrometric method capable of measuring the malodorous boar taint compounds androstenone and skatole in fat samples from male pig carcasses. The developed method is suited for use in commercial abattoirs as an at-line method to detect the presence of these compounds in carcasses or as a high-speed analysis in laboratories with high sample turnover. The chemical assay is based on salt-assisted liquid-liquid extraction and direct measurement with Laser Diode Thermal Desorption-Tandem Mass Spectrometry (LDTD-MS/MS). When fully automated as an at-line method, a single LDTD-MS/MS system will have a measuring capacity of >420 male pig carcasses per hour. The limit of quantification (LOQ) is 0.05 µg/g and 0.10 µg/g for skatole and androstenone, respectively, which is well below the expected sorting thresholds. The reproducibility of the method (%RSD) meets the industry requirement for an RSD of below 10%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|