| Autor: |
Kurt I. Altman, B. Schlegel, T. R. Koszalka, S. Horne |
| Rok vydání: |
1959 |
| Předmět: |
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| Zdroj: |
Biochimica et Biophysica Acta. 35:197-202 |
| ISSN: |
0006-3002 |
| DOI: |
10.1016/0006-3002(59)90348-8 |
| Popis: |
A new method of assay for DNase II was developed based on the reaction of derivatives of desoxyribose with p-NPH. This new method was compared with two other existing procedures for the determination of enzyme activity, i.e., the DPA and u.v. methods. All three procedures were found to be equally accurate. In contrast to the DPA and u.v. methods, the p-NPH procedure was found suitable for the assay of DNase II in subcellular particulates suspended in hypertonic sucrose. Examination of the assay conditions using the DPA method showed that optimal DNase II activity is obtained at pH 4.9 in the presence of 0.1 M sodium acetate buffer and 0.1 M NaCl. In the absence of NaCl, the maximal activity was somewhat lower and the pH optimum was extended from 4.9 to 4.8–5.6. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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