Immunosuppressive prednisolone enhances early cholangiocarcinoma in Syrian hamsters with liver fluke infection and administration of N-nitrosodimethylamine
| Autor: | Orasa Wonkchalee, Ratchadawan Aukkanimart, Pakkayanee Sudsarn, Kulathida Veteewuthacharn, Amornrat Juasook, N. Namwat, Zhiliang Wu, Watcharin Loilome, Pranee Sriraj, Thidarut Boonmars |
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| Rok vydání: | 2011 |
| Předmět: |
Liver Cirrhosis
Cancer Research Pathology medicine.medical_specialty animal diseases Prednisolone Hamster Inflammation Opisthorchiasis Pathology and Forensic Medicine Dimethylnitrosamine Pathogenesis Cholangiocarcinoma Immune system Immunity Cricetinae parasitic diseases medicine Animals Opisthorchis viverrini Cocarcinogenesis biology Mesocricetus Liver Neoplasms General Medicine Liver fluke biology.organism_classification Immunohistochemistry Cell Transformation Neoplastic Oncology Bile Duct Neoplasms Liver Cyclooxygenase 2 Immunology Chronic Disease medicine.symptom Immunosuppressive Agents |
| Zdroj: | Pathology oncology research : POR. 19(1) |
| ISSN: | 1532-2807 |
| Popis: | Chronic infection with Opisthorchis viverrini for many years has been associated with the development of hepatobiliary diseases including cholangiocarcinoma. It is well known that inflammation is a key component of the tumor microenvironment, and that chronic inflammation plays an important role in tumorigenesis. Therefore, in this study cholangiocarcinogenesis was induced in Syrian hamsters in order to observe the cancer-related inflammation. The Syrian hamsters were divided into 5 groups: uninfected controls; normal Syrian hamsters infected with O. viverrini (OV); immunosuppressed Syrian hamsters infected with O. viverrini (OVis); normal Syrian hamsters infected with O. viverrini and administered N-nitrosodimethylamine (CCA); and immunosuppressed Syrian hamsters infected with O. viverrini and administered N-nitrosodimethylamine (CCAis). Syrian hamster livers were later observed for gross pathology and histopathological changes; COX2 was analyzed by immunohistochemical staining. We found a decreased number of inflammatory cells surrounding the hepatic bile duct in the OVis group, but not in the OV and CCAis groups. However, in the CCAis group (with suppressed immunity) early appearance and greater severity of cholangiocarcinoma were observed; gross pathological examination revealed many cancer nodularities on the liver surface, and histopathological studies showed the presence of cancer cells, findings which correlated with the predominant expression of COX2. The present study suggests that host immune responses are intended to ameliorate pathology, and they are also crucially associated with pathogenesis in O. viverrini infection; the unbalancing of host immunity may enhance cancer-related inflammation. |
| Databáze: | OpenAIRE |
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