Enhancement of bone mineral density and body mass in newborn chickens by in ovo injection of ionic-hydroxyapatite nanoparticles of bacterial origin
| Autor: | Elham Ahmadzadeh, Mehrdad Mashkour, Farid Talebnia Rowshan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Bone density medicine.medical_treatment 0206 medical engineering Biomedical Engineering Biophysics Bioengineering 02 engineering and technology Kidney In ovo Injections Biomaterials Andrology Calcification Physiologic Bone Density medicine Animals Humans Saline Ions Bone mineral Body Weight Embryo Enterobacter aerogenes 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering Durapatite medicine.anatomical_structure Liver Toxicity MCF-7 Cells Nanoparticles 0210 nano-technology Chickens Calcification |
| Zdroj: | Journal of Materials Science: Materials in Medicine. 30 |
| ISSN: | 1573-4838 0957-4530 |
| Popis: | Using non-drug, non-surgical treatments for improving bone mineral diseases in newborn babies is an important topic for neonatologists. The present study introduces bacterial synthesized ionic nano-hydroxyapatite (Bio-HA) for the development of bone mineral density in the chicken embryo model. In vitro cytotoxicity analyses were demonstrated the optimal concentrations of Bio-HA compared to a chemically-synthesized hydroxyapatite (Ch-HA). Toxicity of Bio-HA on MCF-7 human cell lines was negligible at the concentrations less than 500 μg/mL whereas Ch-HA showed similar results at the concentrations less than 100 μg/mL. Therefore, concentrations at 50 μg/mL and 100 μg/mL were selected for in ovo injection of both materials into the fertilized eggs. The newly hatched chickens were sacrificed in order to monitor their serological factors, total body mass, bone mineral contents and bone mineral density. The results confirmed that Bio-HA increased the average body weight and bone mineral indices of chickens in comparison to the Ch-HA and negative controls (normal saline and intact groups). In view of the intact group, no liver or kidney damage occurred in the groups receiving Bio-HA which promises the effectiveness of these nanoparticles for the treatment of afterbirth bone mineral deficiency. |
| Databáze: | OpenAIRE |
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