The sickle cell mouse lung: pro-inflammatory and primed for allergic inflammation
| Autor: | Biree Andemariam, Roger S. Thrall, Linda A. Guernsey, Anurag Singh, Alexander J. Adami, Eric R. Secor, Jeffrey T. McNamara |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Chemokine
Regulatory T cell Ovalbumin T-Lymphocytes Anemia Sickle Cell Article Allergic inflammation Leukocyte Count Immune system Physiology (medical) hemic and lymphatic diseases medicine Hypersensitivity CXCL10 Animals Interferon gamma Interleukin 5 Lung Hemizygote Inflammation biology Biochemistry (medical) Public Health Environmental and Occupational Health General Medicine respiratory system Asthma Eosinophils Mice Inbred C57BL Disease Models Animal Mucus medicine.anatomical_structure Immunology biology.protein Cytokines Female Bronchial Hyperreactivity Bronchoalveolar Lavage Fluid CD8 medicine.drug |
| Popis: | Comorbid asthma in sickle cell disease (SCD) confers higher rates of vaso-occlusive pain and mortality, yet the physiological link between these two distinct diseases remains puzzling. We used a mouse model of SCD to study pulmonary immunology and physiology before and after the induction of allergic airway disease (AAD). SCD mice were sensitized with ovalbumin (OVA) and aluminum hydroxide by the intraperitoneal route followed by daily, nose-only OVA-aerosol challenge to induce AAD. The lungs of naive SCD mice showed signs of inflammatory and immune processes: (1) histologic and cytochemical evidence of airway inflammation compared with naive wild-type mice; (2) bronchoalveolar lavage (BAL) fluid contained increased total lymphocytes, %CD8+ T cells, granulocyte-colony stimulating factor, interleukin 5 (IL-5), IL-7, and chemokine (C-X-C motif) ligand (CXCL)1; and (3) lung tissue and hilar lymph node (HLN) had increased CD4+, CD8+, and regulatory T (Treg) cells. Furthermore, SCD mice at AAD demonstrated significant changes compared with the naive state: (1) BAL fluid with increased %CD4+ T cells and Treg cells, lower %CD8+ T cells, and decreased interferon gamma, CXCL10, chemokine (C-C motif) ligand 2, and IL-17; (2) serum with increased OVA-specific immunoglobulin E, IL-6, and IL-13, and decreased IL-1α and CXCL10; (3) no increase in Treg cells in the lung tissue or HLN; and (4) hyporesponsiveness to methacholine challenge. In conclusion, SCD mice have an altered immunologic pulmonary milieu and physiological responsiveness. These findings suggest that the clinical phenotype of AAD in SCD mice differs from that of wild-type mice and that individuals with SCD may also have a unique, divergent phenotype perhaps amenable to a different therapeutic approach. |
| Databáze: | OpenAIRE |
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