Additional file 3 of A novel epitope-blocking ELISA for specific and sensitive detection of antibodies against H5-subtype influenza virus hemagglutinin

Autor: Sączyńska, Violetta, Florys-Jankowska, Katarzyna, Porębska, Anna, Cecuda-Adamczewska, Violetta
Rok vydání: 2021
DOI: 10.6084/m9.figshare.14524603.v1
Popis: Additional file 3. Development and optimization of H5 EB-ELISA. Fig. S1. The ELISA titration curves of G-7-27-18 mAb against rH5-BEVS coated on the well surfaces with varied hydrophilicity. Titration curves were denoted according to the plate type. Fig. S2. The ELISA titration curves of G-7-27-18 mAb against rH5-BEVS under preliminary assay conditions. rH5-BEVS was coated at (A) 1.0 μg/mL or (B) 0.5 μg/mL on MediSorp plates. Titration curves were denoted according to the coating concentration of rH5-BEVS in μg per mL (1 μg or 0.5 μg) and then the time of plate incubation with G-7-27-18 mAb (60 min or 30 min), anti-mouse antibodies (60 min or 30 min) at the indicated dilution (1:1,000) and TMB (15 min or 10 min). Fig. S3. The ELISA titration curves of G-7-27-18 mAb against rH5-BEVS under assay conditions optimized in step 1. Titration curves were denoted according to the coating concentration of rH5-BEVS in μg per mL (0.5 μg) and then the time of plate incubation with G-7-27-18 mAb (60 min or 30 min), anti-mouse antibodies (60 min) at the indicated dilutions (1:1,000, 1:1,500, 1:2,000 or 1:2,500) and TMB (15 min). Fig. S4. The ELISA titration curves of G-7-27-18 mAb against rH5-BEVS under assay conditions optimized in step 2. Titration curves were denoted according to the coating concentration of rH5-BEVS in μg per mL (0.5 μg) and then the time of plate incubation with G-7-27-18 mAb (60 min or 30 min), anti-mouse antibodies (60 min) at the indicated dilutions (1:1,500, 1:2,000, 1:3,000 or 1:4,000) and TMB (15 min). Fig. S5. The ELISA titration curves of G-7-27-18 mAb against rH5-BEVS under assay conditions optimized in step 3. Titration curves were denoted according to the coating concentration of rH5-BEVS in μg per mL (0.5 μg) and then the time of plate incubation with G-7-27-18 mAb (60 min), anti-mouse antibodies (60 min) at the indicated dilutions (1:2,500, 1:3,000, 1:3,500 or 1:4,000) and TMB (15 min).
Databáze: OpenAIRE