A novel method using blinatumomab for efficient, clinical-grade expansion of polyclonal T cells for adoptive immunotherapy
| Autor: | Sabrina Cribioli, Rachele Alzani, Martino Introna, Michela Bonzi, Rut Valgardsdottir, Maria Chiara Finazzi, Enrico Pesenti, Clara Albanese, Anna E. D’Amico, Gianmaria Borleri, Dirk Nagorsen, Alessandro Rambaldi, Josée Golay, Giulia Quaresmini |
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| Rok vydání: | 2014 |
| Předmět: |
Cytotoxicity
Immunologic T cell medicine.medical_treatment Chronic lymphocytic leukemia Immunology Programmed Cell Death 1 Receptor Cell Culture Techniques Immunotherapy Adoptive Immunophenotyping Mice Antigen T-Lymphocyte Subsets hemic and lymphatic diseases Cell Line Tumor Antibodies Bispecific Immunology and Allergy Medicine Cytotoxic T cell Animals Humans business.industry Immunotherapy medicine.disease Leukemia Lymphocytic Chronic B-Cell Disease Models Animal medicine.anatomical_structure Phenotype Antigens Surface Interleukin-2 Blinatumomab Female business CD8 medicine.drug |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 193(9) |
| ISSN: | 1550-6606 |
| Popis: | Current treatment of chronic lymphocytic leukemia (CLL) patients often results in life-threatening immunosuppression. Furthermore, CLL is still an incurable disease due to the persistence of residual leukemic cells. These patients may therefore benefit from immunotherapy approaches aimed at immunoreconstitution and/or the elimination of residual disease following chemotherapy. For these purposes, we designed a simple GMP-compliant protocol for ex vivo expansion of normal T cells from CLL patients’ peripheral blood for adoptive therapy, using bispecific Ab blinatumomab (CD3 × CD19), acting both as T cell stimulator and CLL depletion agent, and human rIL-2. Starting from only 10 ml CLL peripheral blood, a mean 515 × 106 CD3+ T cells were expanded in 3 wk. The resulting blinatumomab-expanded T cells (BET) were polyclonal CD4+ and CD8+ and mostly effector and central memory cells. The Th1 subset was slightly prevalent over Th2, whereas Th17 and T regulatory cells were |
| Databáze: | OpenAIRE |
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