X-ray induced reduction of the crystal of high-molecular-weight cytochromecrevealed by microspectrophotometry
| Autor: | Midori Sato, Kiyoshi Ozawa, Yukio Morimoto, Naoki Shibata, Yuki Takayama, Hideo Akutsu, Noritake Yasuoka, Yoshiki Higuchi |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Models
Molecular Nuclear and High Energy Physics Radiation Absorption spectroscopy biology Protein Conformation Chemistry X-Rays Cytochrome c X-ray Cytochromes c Synchrotron radiation Crystal structure biology.organism_classification Crystal Crystallography X-Ray Diffraction Microspectrophotometry biology.protein Computer Simulation Desulfovibrio vulgaris Irradiation Instrumentation Synchrotrons |
| Zdroj: | Journal of Synchrotron Radiation. 11:113-116 |
| ISSN: | 0909-0495 |
| Popis: | The crystal structures of high-molecular-weight cytochrome c (HMC) from Desulfovibrio vulgaris Hildenborough in the transient and reduced states have been determined at 2.8 A resolution. An absorption spectrum measured with microspectrophotometer indicated that about 86% of the hemes were reduced after 45 min irradiation of the X-ray beam. Further exposure for 90 min did not significantly change the spectrum. These results suggest that HMC in the crystalline state is easily reduced by illumination of the X-ray beam from synchrotron radiation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|