Identification and quantification of free radicals during myocardial ischemia and reperfusion using electron paramagnetic resonance spectroscopy
| Autor: | Catherine Vergely, Gaëlle Clermont, Véronique Maupoil, Antoine Bril, Luc Rochette |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Free Radicals
Radical Myocardial Ischemia Biophysics Myocardial Reperfusion Myocardial Reperfusion Injury Ascorbic Acid Nitric Oxide medicine.disease_cause Photochemistry Biochemistry Redox law.invention Nitric oxide chemistry.chemical_compound Nuclear magnetic resonance law Freezing medicine Animals Humans Electron paramagnetic resonance Spectroscopy Molecular Biology Spin trapping Chemistry Electron Spin Resonance Spectroscopy medicine.disease Oxidative Stress Oxidation-Reduction Spin Trapping Reperfusion injury Oxidative stress |
| Zdroj: | Archives of Biochemistry and Biophysics. 420:209-216 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/j.abb.2003.07.007 |
| Popis: | There is general agreement that free radicals are involved in reperfusion injury. Electron paramagnetic resonance (EPR) spectroscopy can be considered as the more suitable technique to directly measure and characterize free radical generation during myocardial ischemia and reperfusion. There are essentially two approaches used in the detection of unstable reactive species: freezing technique and spin traps. The detection of secondary free radicals or ascorbyl free radicals during reperfusion might provide an index of oxidative stress. Spin trapping can also characterize nitric oxide. EPR spectroscopy can provide important data regarding redox state and free radical metabolism but ideally, the spin traps must not interfere with cell or organism function. |
| Databáze: | OpenAIRE |
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