Effect of vitamin A supplementation at different gaseous environments on in vitro development of pre-implantation sheep embryos to the blastocyst stage
| Autor: | M. B. Shankar, N. Rajesh, M. Deecaraman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Vitamin
In vitro fertilisation medicine.medical_treatment Embryogenesis Retinol Embryo culture Embryo Biology in vitro fertilisation SF1-1100 vitamin A Animal culture Andrology embryo culture chemistry.chemical_compound ovine medicine.anatomical_structure Human fertilization chemistry Biochemistry embryonic structures medicine oxidative stress Animal Science and Zoology Blastocyst |
| Zdroj: | Animal, Vol 4, Iss 11, Pp 1884-1890 (2010) |
| ISSN: | 1751-7311 |
| Popis: | Vitamin A (all-trans retinol) is an important antioxidant whose role in embryo development in vitro and in vivo is well established. Oxidative stress is a major cause of defective embryo development. This study evaluated the effects of all-trans retinol supplementation to maturation and embryo culture media under different gaseous environments on the development of ovine oocytes and embryos in vitro. The percentages of cleavage, morula and blastocyst, total cell count and comet assay were taken as indicators of developmental competence of embryos. In experiments I and II, all-trans retinol at concentrations of 0, 2, 4, 6, 8 and 10 μM were supplemented to the oocyte maturation medium and cultured in an environment of 5% or 20% O2 respectively. All-trans retinol supplementation (6 μM) to the maturation medium at 5% O2 levels significantly increased blastocyst yield and total cell number (P < 0.05). Maturation of oocytes in a 20% O2 environment bettered cleavage rates in the 6 μM supplemented group compared with the control group (P < 0.05). In experiments III and IV, all-trans retinol, at the aforesaid concentrations was supplemented to embryo culture media under a 5% or 20% O2 environment, respectively. All-trans retinol supplementation to the embryo culture medium at 5% O2 levels did not yield any significant result whereas the culture at 20% O2 levels gave significantly higher blastocyst yield in the 6 μM supplemented group compared with the control group (P < 0.01). |
| Databáze: | OpenAIRE |
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