Orabase formulation with cashew gum polysaccharide decreases inflammatory and bone loss hallmarks in experimental periodontitis
| Autor: | Hygor Ferreira Fernandes, Luiz Felipe de Carvalho França, Giovanny R. Pinto, Daniel Fernando Pereira Vasconcelos, Felipe Rodolfo Pereira da Silva, David Di Lenardo, Durcilene Alves da Silva, Ana Carolina M. Leódido, Jand V.R. Medeiros, Manoel Dias de Souza Filho |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.drug_class medicine.medical_treatment Interleukin-1beta Alveolar Bone Loss Pharmacology Polysaccharide Biochemistry Anti-inflammatory 03 medical and health sciences 0302 clinical medicine Structural Biology Polysaccharides medicine Animals Humans Anacardium Rats Wistar Periodontitis Molecular Biology Saline Dental alveolus Peroxidase chemistry.chemical_classification Inflammation biology Tumor Necrosis Factor-alpha RANK Ligand 030206 dentistry General Medicine medicine.disease biology.organism_classification Rats Disease Models Animal Oxidative Stress 030104 developmental biology chemistry Gene Expression Regulation RANKL Carboxymethylcellulose Sodium Immunology biology.protein Female Adjuvant |
| Zdroj: | International journal of biological macromolecules. 107 |
| ISSN: | 1879-0003 |
| Popis: | This study aimed to investigate the chemical characteristics and the effects of an orabase gel with Cashew Gum Polysaccharide (CG-P) from Anacardium occidentale L. on alveolar bone loss and relative mRNA expression of TNF-α, IL-1β, RANK, RANKL, and OPG in the periodontal tissue of Wistar rats (Rattus norvegicus) subjected to ligature-induced periodontitis. Crude cashew gum was collected and purified by chemical processes; then, the CG-P was mixed with orabase gel. Female rats were randomly divided into four groups of six animals each: saline 0.9% (Sal Group); orabase gel (Gel Group); 50mg CG-P/1g orabase gel (CG-P50 Group) and 150mg CG-P/1g orabase gel (CG-P150 Group). Periodontitis was induced in the animals; they were treated for 20days with one daily topical application. The purification process of CG-P presented high yield and resulted in a protein-free product. The treatment with CG-P150 (150mg CG-P/1g orabase gel) significantly reduced alveolar bone loss, decreased the relative mRNA expression of TNF-α, IL-1β, RANKL and the RANKL/OPG ratio, and caused a significant decrease in myeloperoxidase activity of the gingival tissue. Thus, the CG-P in orabase represents a potential adjuvant drug for the treatment of periodontitis and possible source of new biotechnological discoveries. |
| Databáze: | OpenAIRE |
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