Novel Human Radiation Exposure Biomarker Panel Applicable for Population Triage
| Autor: | Jose G. Bazan, Lei Shura, Shirley Lee, Susan J. Knox, Hua Lin, Thomas A. Shaler, M. Travis Harrison, David E. Cooper, Polly Y. Chang, Annalisa D'Andrea, Robert Balog, Lucy E. Schoen |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Oncology Cancer Research medicine.medical_specialty Pathology Transplantation Conditioning Lymphoma Point-of-Care Systems medicine.medical_treatment Population Down-Regulation Infections Radiation Dosage Ionizing radiation Young Adult Internal medicine Humans Medicine Radiology Nuclear Medicine and imaging Saliva education Aged Aged 80 and over education.field_of_study Leukemia Lymphatic Irradiation Radiation business.industry Confounding Hematopoietic Stem Cell Transplantation Tenascin Total body irradiation Blood proteins Up-Regulation Radiation therapy Transplantation Clusterin Case-Control Studies Myelodysplastic Syndromes Amylases Wounds and Injuries Female False positive rate Triage business Biomarkers Whole-Body Irradiation |
| Zdroj: | International Journal of Radiation Oncology*Biology*Physics. 90:612-619 |
| ISSN: | 0360-3016 |
| DOI: | 10.1016/j.ijrobp.2014.05.046 |
| Popis: | Purpose To identify a panel of radiation-responsive plasma proteins that could be used in a point-of-care biologic dosimeter to detect clinically significant levels of ionizing radiation exposure. Methods and Materials Patients undergoing preparation for hematopoietic cell transplantation using radiation therapy (RT) with either total lymphoid irradiation or fractionated total body irradiation were eligible. Plasma was examined from patients with potentially confounding conditions and from normal individuals. Each plasma sample was analyzed for a panel of 17 proteins before RT was begun and at several time points after RT exposure. Paired and unpaired t tests between the dose and control groups were performed. Conditional inference trees were constructed based on panels of proteins to compare the non-RT group with the RT group. Results A total of 151 patients (62 RT, 41 infection, 48 trauma) were enrolled on the study, and the plasma from an additional 24 healthy control individuals was analyzed. In comparison with to control individuals, tenascin-C was upregulated and clusterin was downregulated in patients receiving RT. Salivary amylase was strongly radiation responsive, with upregulation in total body irradiation patients and slight downregulation in total lymphoid irradiation patients compared with control individuals. A panel consisting of these 3 proteins accurately distinguished between irradiated patients and healthy control individuals within 3 days after exposure: 97% accuracy, 0.5% false negative rate, 2% false positive rate. The accuracy was diminished when patients with trauma, infection, or both were included (accuracy, 74%-84%; false positive rate, 14%-33%, false negative rate: 8%-40%). Conclusions A panel of 3 proteins accurately distinguishes unirradiated healthy donors from those exposed to RT (0.8-9.6 Gy) within 3 days of exposure. These findings have significant implications in terms of triaging individuals in the case of nuclear or other radiologic events. |
| Databáze: | OpenAIRE |
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