In vitro alpha-amylase inhibition and in vivo antioxidant potential of Amaranthus spinosus in alloxan-induced oxidative stress in diabetic rats
| Autor: | P.A. Arun Kumar, B. Manoj, B.S. Ashok Kumar, R. Nandeesh, K. Lakshman, Vinod Kumar, D. Sheshadri Shekar |
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| Rok vydání: | 2010 |
| Předmět: |
Antioxidant
medicine.medical_treatment Blood sugar Pharmacology Alpha amylase medicine.disease_cause chemistry.chemical_compound Amaranthus spinosus Diabetic Alloxan Medicine biology Agricultural and Biological Sciences(all) business.industry Glutathione Malondialdehyde Biochemistry chemistry Catalase biology.protein Original Article General Agricultural and Biological Sciences business Alpha-amylase Oxidative stress |
| Zdroj: | Saudi journal of biological sciences. 18(1) |
| ISSN: | 1319-562X |
| Popis: | Amaranthus spinosus Linn. (Amaranthaceae), commonly known as “Mulluharivesoppu” in Kannada, is used in the Indian traditional system of medicine for the treatment of diabetes. The present study deals with the scientific evaluation of alpha amylase and the antioxidant potential of methanol extract of A. spinosus (MEAS). The aim of this study was to investigate in vitro alpha-amylase enzyme inhibition by CNPG3 (2-chloro-4-nitrophenol α-d-maltotrioside) and in vivo antioxidant potential of malondialdehyde (MDA), glutathione (GSH), catalase (CAT) and total thiols (TT) in alloxan-induced diabetic rats of a methanolic extract of A. spinosus. Blood sugar was also determined in MEAS-treated alloxan-induced diabetic rats. MEAS showed significant inhibition of alpha-amylase activity and IC50 46.02μg/ml. Oral administration of MEAS (200 and 400mg/kg) for 15days showed significant reduction in the elevated blood glucose, MDA and restores GSH, CAT and TT levels as compared with a diabetic control. The present study provides evidence that the methanolic extract of A. spinosus has potent alpha amylase, anti-diabetic and antioxidant activities. |
| Databáze: | OpenAIRE |
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