Changes in the Contractile Activity and Reactivity to 5-HT of Smooth Muscles of Rats Following Total Body Irradiation with Accelerated Electrons
| Autor: | Marieta V Sopadzhieva, Athanas D. Krustev, Valentin I. Turiyski, Zhanet Grudeva-Popova, N. Prissadova, Raina G. Ardasheva, Veselin G Popov, Mariya I Vlaykova, Xenodochidis A Charilaos |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Serotonin gastric smooth muscles lcsh:Medicine Electrons Serotonergic Tonic (physiology) Contractility 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine accelerated electron beam medicine Animals Rats Wistar Neurotransmitter 5-HT receptor lcsh:R Muscle Smooth General Medicine Total body irradiation Rats 030104 developmental biology Endocrinology chemistry 030211 gastroenterology & hepatology Whole-Body Irradiation Hormone Muscle Contraction |
| Zdroj: | Folia Medica, Vol 61, Iss 3, Pp 411-418 (2019) |
| ISSN: | 1314-2143 |
| Popis: | Background: Besides its “classical” neurotransmitter function in the central and peripheral nervous systems, serotonin, or 5-hydroxytryptamine (5-HT) is also a local hormone in a number of tissues, including those of the GI tract. Radiation is known to be able to disrupt certain functions of the tract, modulated by 5-HT-signaling pathways, or the serotonin receptors themselves. Aim: The present investigation focused on clarifying the nature and extent of influence of an accelerated electron beam with energy of 9 MeV on the serotonergic mediation of healthy smooth muscle gastric tissue of rats following total body irradiation of the animals. Materials and methods: The study involved a control group and two experimental groups of animals exposed to 1 and 5 Gy, respectively, using Siemens Primus S/N 3561. Circular smooth muscle tissues were isolated from rats 1 hour and 18 hours after they were exposed to 1 and 5 Gy and also 5 days after irradiation from the rats that received a dose of 5 Gy in order to investigate the action of exogenous serotonin at increasing concentrations from 10-8 to 10-4 mol/l. The contractile reactivity of each group SM preparations was registered isometrically. Results: Electron beams with energy of 9 MeV did not damage the contractile apparatus of gastric SM of rats and had a stimulating effect on contractility resulting from rapidly developing processes (1 hour) or later occurring once (5 days). Conclusions: Difference was observed in the importance of the factors of received dose, lapse of time from irradiation to investigation of SM tissues, and exogenous 5-HT concentration for the changes in SM reactivity in serotonin-induced tonic and phasic responses. |
| Databáze: | OpenAIRE |
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