Expression and cellular localisation of diamine oxidase in the gastrointestinal tract of pigs
| Autor: | H. Maier, E. Bodner, H. G. Schwelberger, B. Stalzer |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Swine
Immunology Immunoblotting Kidney chemistry.chemical_compound Cellular localisation Animals Pharmacology chemistry.chemical_classification Gastrointestinal tract biology Epithelial Cells Immunohistochemistry Intestines Subcellular distribution Enzyme chemistry Biochemistry Microscopy Fluorescence biology.protein Electrophoresis Polyacrylamide Gel Female Amine Oxidase (Copper-Containing) Antibody Diamine oxidase Digestive System Histamine |
| Zdroj: | Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 47 |
| ISSN: | 1023-3830 |
| Popis: | Diamine oxidase (DAO, EC 1.4.3.6) is the major enzyme degrading histamine and other biogenic amines [1, 2]. Although DAO activity has been reported for various mammalian tissues, very little is known about the cellular and subcellular distribution of the enzyme. Therefore, we employed a combination of DAO activity assays, immunoblotting with an anti-DAO antibody, and immunohistochemical staining to localise DAO in the gastrointestinal tract of pigs. |
| Databáze: | OpenAIRE |
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