Competitive particle concentration fluorescence immunoassays for measuring anti-diabetic drug levels in mouse plasma
| Autor: | Stuart W. Bright, Frank C. Tinsley, Gerald Gold, Lora Louise Fitch, Schmiegel Klaus K, Samuel J. Dominianni |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
medicine.drug_class Immunology Biological Availability Mice Inbred Strains Tuberculin Monoclonal antibody Antibodies Fluorescence Mice Troglitazone Antibody Specificity medicine Animals Hypoglycemic Agents Immunology and Allergy Chromans Bovine serum albumin Receptor Immunoassay Mice Inbred BALB C Chromatography Molecular Structure biology medicine.diagnostic_test Chemistry Antibodies Monoclonal Phycoerythrin Bioavailability Thiazoles Glucose Biochemistry biology.protein Thiazolidinediones Drug Monitoring Antibody Pharmacophore medicine.drug |
| Zdroj: | Journal of Immunological Methods. 207:23-31 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/s0022-1759(97)00088-4 |
| Popis: | Two competitive particle concentration fluorescence immunoassays were developed to measure blood levels of analogs of anti-diabetic drugs being tested in diabetic mice. Ligands that contained the active pharmacophores were conjugated to PPD for immunization and to beta-phycoerythrin for use as a tracer in the immunoassays. Approximately 90% of 262 compounds assayed were detectable at less than 120 nM in plasma which was well below the estimated therapeutic level of 1 microM for lowering blood glucose. These data were used to define the bioavailability of test compounds and assist in decisions of constructing active analogs. Of additional interest, we noted crossreactivity of one monoclonal antibody for 3 different compound classes that are all known to bind with varying affinities to peroxisome proliferator-activated receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect