The Ethanol Extract of Marine Sponge Aaptos suberitoides Suppress Cell Viability, Cell Proliferation and Cell Migration in HER2-Positive Breast Cancer Cell Line
| Autor: | Tenny Putri, Fadli Zaky Arsydinilhuda, Nurul Qomarilla, Maman Abdurahman, Eko Fuji Ariyanto, Harold Eka Atmaja, Edy Meiyanto, Yunisa Pamela, Ryzkianty Annis Nurdin, Fathul Huda, Annisa Dewi Nugrahani, Ajeng Kartika, Beginer Subhan, Ikhwan Resmala Sudji, Re Septian Ilhamsyah, Hermin Aminah Usman, Muhammad Hasan Bashari |
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| Rok vydání: | 2021 |
| Předmět: |
Ethanol
Receptor ErbB-2 Tissue Extracts Cell growth Apoptosis Breast Neoplasms General Medicine Molecular biology Porifera chemistry.chemical_compound chemistry Cell Movement Cell culture Tumor Cells Cultured Animals Humans Female MTT assay Viability assay Cytotoxicity Clonogenic assay IC50 Cell Proliferation |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 22:25-32 |
| ISSN: | 2476-762X |
| DOI: | 10.31557/apjcp.2021.22.s1.25 |
| Popis: | Objective This study aimed to investigate the cytotoxicity, anti-proliferation and anti-migration effect of the ethanol extract of Aaptos suberitoides on trastuzumab-resistant HER2+ breast cancer cell line. Methods Aaptos suberitoides was collected from Tinjil Island, Banten, Indonesia, and was processed with maceration and ethanol extraction. HCC-1954 cells were treated with the ethanol extract and then followed by 3- [4, 5-dimethylthiazol-2-yl] -2.5 diphenyl tetrazolium bromide (MTT) assay to assess cytotoxicity, clonogenic assay and three-dimensional (3D) spheroid assay to evaluate anti-proliferative effect in two-dimensional and 3D model, respectively, and wound healing assay to determine anti-cell migration effect. Four parametric regression was used to analyse the IC50. Results This study revealed that the ethanol extract of Aaptos suberitoides suppressed cell viability in correlation with cell death induction. The IC50 values of the ethanol extract of Aaptos suberitoides using MTT assay and clonogenic assay were 12.0 ppm and 4.36 ppm, respectively. The extract demonstrated an inhibition effect on spheroid growth. In low concentration, the extract of Aaptos suberitoides inhibited cell migration. Furthermore, MS analysis showed that the most abundant compounds in this extract has molecular weight m/z 229.81 [M+H]+. Conclusion This study revealed that the ethanol extract of Aaptos suberitoides demonstrates cytotoxicity, anti-proliferation and anti-migration effect as well as inhibition effect on three-dimensional spheroid growth in trastuzumab-resistant HER2+ breast cancer cell line. . |
| Databáze: | OpenAIRE |
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